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The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure

OBJECTIVE: To confirm the role of X-ray exposure in treating infected wound fractures at the lower limb and determine X-ray exposure times. METHODS: Fifty-one wound swabs were collected from patients with infected open fractures at the lower limb with grade II, IIIA, B, and C according to the Gustil...

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Autores principales: Mahdi, Ali A., Al-Salmani, Tuqa S., Al-Qaisi, Mustafa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106931/
https://www.ncbi.nlm.nih.gov/pubmed/32256601
http://dx.doi.org/10.1155/2020/3129356
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author Mahdi, Ali A.
Al-Salmani, Tuqa S.
Al-Qaisi, Mustafa M.
author_facet Mahdi, Ali A.
Al-Salmani, Tuqa S.
Al-Qaisi, Mustafa M.
author_sort Mahdi, Ali A.
collection PubMed
description OBJECTIVE: To confirm the role of X-ray exposure in treating infected wound fractures at the lower limb and determine X-ray exposure times. METHODS: Fifty-one wound swabs were collected from patients with infected open fractures at the lower limb with grade II, IIIA, B, and C according to the Gustilo and Anderson classification system and then cultured. The bacterial isolates were identified by biochemical tests and the VITEK-2 System and tested against several antibiotics. The X-ray exposure was done for open fractures by radiography (at kV133 and 5 milliambers). RESULTS: The higher isolation rate was recorded for Staphylococcus aureus with 21 (41.2%) isolates, and most of them (20, 95.2%) were isolated from grade II fractures. The isolation rate of Gram-negative bacteria was 25.5% for Escherichia coli with 13 isolates, 19.6% for Pseudomonas aeruginosa with 10 isolates, and 13.7% for Klebsiella pneumoniae with 7 isolates, most of which were isolated from grade III fractures. The isolation rate of P. aeruginosa was 60% (6 isolates) from grade IIIA and 71.4% (5 isolates) from grade IIIB for K. pneumoniae, while for E. coli it was 69.2% (9 isolates) from grade IIIC. All the bacterial isolates recorded high levels of antibiotic resistance against most tested antibiotics. Wound cultures of grade II fractures appeared sterile after the first X-ray exposure, and these wounds were infected with S. aureus or P. aeruginosa. However, cultures of grade IIIA and IIIB fractures appeared sterile after the second X-ray exposure for all isolated bacteria, except for S. aureus (grade IIIA fractures) (after the third X-ray exposure). Grade IIIC fractures showed sterile culture after the third X-ray exposure for wounds infected with P. aeruginosa and E. coli. CONCLUSIONS: The study concluded that X-ray exposure showed high effectiveness in treating infected open fractures.
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spelling pubmed-71069312020-04-02 The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure Mahdi, Ali A. Al-Salmani, Tuqa S. Al-Qaisi, Mustafa M. Int J Microbiol Research Article OBJECTIVE: To confirm the role of X-ray exposure in treating infected wound fractures at the lower limb and determine X-ray exposure times. METHODS: Fifty-one wound swabs were collected from patients with infected open fractures at the lower limb with grade II, IIIA, B, and C according to the Gustilo and Anderson classification system and then cultured. The bacterial isolates were identified by biochemical tests and the VITEK-2 System and tested against several antibiotics. The X-ray exposure was done for open fractures by radiography (at kV133 and 5 milliambers). RESULTS: The higher isolation rate was recorded for Staphylococcus aureus with 21 (41.2%) isolates, and most of them (20, 95.2%) were isolated from grade II fractures. The isolation rate of Gram-negative bacteria was 25.5% for Escherichia coli with 13 isolates, 19.6% for Pseudomonas aeruginosa with 10 isolates, and 13.7% for Klebsiella pneumoniae with 7 isolates, most of which were isolated from grade III fractures. The isolation rate of P. aeruginosa was 60% (6 isolates) from grade IIIA and 71.4% (5 isolates) from grade IIIB for K. pneumoniae, while for E. coli it was 69.2% (9 isolates) from grade IIIC. All the bacterial isolates recorded high levels of antibiotic resistance against most tested antibiotics. Wound cultures of grade II fractures appeared sterile after the first X-ray exposure, and these wounds were infected with S. aureus or P. aeruginosa. However, cultures of grade IIIA and IIIB fractures appeared sterile after the second X-ray exposure for all isolated bacteria, except for S. aureus (grade IIIA fractures) (after the third X-ray exposure). Grade IIIC fractures showed sterile culture after the third X-ray exposure for wounds infected with P. aeruginosa and E. coli. CONCLUSIONS: The study concluded that X-ray exposure showed high effectiveness in treating infected open fractures. Hindawi 2020-03-19 /pmc/articles/PMC7106931/ /pubmed/32256601 http://dx.doi.org/10.1155/2020/3129356 Text en Copyright © 2020 Ali A. Mahdi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mahdi, Ali A.
Al-Salmani, Tuqa S.
Al-Qaisi, Mustafa M.
The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title_full The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title_fullStr The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title_full_unstemmed The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title_short The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure
title_sort novel role of healing from bacterial infections of lower limb open fractures by x-ray exposure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106931/
https://www.ncbi.nlm.nih.gov/pubmed/32256601
http://dx.doi.org/10.1155/2020/3129356
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