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miR-95 promotes osteosarcoma growth by targeting SCNN1A
Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long-term prognosis remains poor. miRNAs are non-coding single-stranded RNAs ~22 nucleotides i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107771/ https://www.ncbi.nlm.nih.gov/pubmed/32323794 http://dx.doi.org/10.3892/or.2020.7514 |
Sumario: | Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long-term prognosis remains poor. miRNAs are non-coding single-stranded RNAs ~22 nucleotides in length. Increasing evidence suggests that numerous miRNAs may play critical roles in tumorigenesis and tumor progression; however, the role of miR-95 in OS has not been examined. In the present study, we investigated the role of miR-95 in OS using in vitro and in vivo models and publicly available expression data. Our findings indicate that abnormal miR-95 expression occurs in OS, according to the Gene Expression Omnibus (GEO) database. The miR-95 inhibitor reduced cell proliferation and promoted apoptosis in OS cell lines as detected by EdU staining, TUNEL staining and flow cytometry. Furthermore, a dual luciferase reporter assay revealed that miR-95 regulates the cell cycle of OS cells and apoptosis by targeting sodium channel epithelial 1α subunit (SCNN1A). Additionally, miR-95 antagomir suppressed the growth of U2OS xenograft tumors in a mouse model. In summary, our results suggest that miR-95 induces OS growth in vitro and in vivo by targeting SCNN1A. Our results help clarify the mechanism underlying the miR-95-mediated effects on OS tumor growth, thus potentially establishing it as a diagnostic target. |
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