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miR-95 promotes osteosarcoma growth by targeting SCNN1A
Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long-term prognosis remains poor. miRNAs are non-coding single-stranded RNAs ~22 nucleotides i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107771/ https://www.ncbi.nlm.nih.gov/pubmed/32323794 http://dx.doi.org/10.3892/or.2020.7514 |
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author | Geng, Yannan Zhao, Shaorong Jia, Yutao Xia, Gang Li, Huiming Fang, Zhao Zhang, Quan Tian, Rong |
author_facet | Geng, Yannan Zhao, Shaorong Jia, Yutao Xia, Gang Li, Huiming Fang, Zhao Zhang, Quan Tian, Rong |
author_sort | Geng, Yannan |
collection | PubMed |
description | Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long-term prognosis remains poor. miRNAs are non-coding single-stranded RNAs ~22 nucleotides in length. Increasing evidence suggests that numerous miRNAs may play critical roles in tumorigenesis and tumor progression; however, the role of miR-95 in OS has not been examined. In the present study, we investigated the role of miR-95 in OS using in vitro and in vivo models and publicly available expression data. Our findings indicate that abnormal miR-95 expression occurs in OS, according to the Gene Expression Omnibus (GEO) database. The miR-95 inhibitor reduced cell proliferation and promoted apoptosis in OS cell lines as detected by EdU staining, TUNEL staining and flow cytometry. Furthermore, a dual luciferase reporter assay revealed that miR-95 regulates the cell cycle of OS cells and apoptosis by targeting sodium channel epithelial 1α subunit (SCNN1A). Additionally, miR-95 antagomir suppressed the growth of U2OS xenograft tumors in a mouse model. In summary, our results suggest that miR-95 induces OS growth in vitro and in vivo by targeting SCNN1A. Our results help clarify the mechanism underlying the miR-95-mediated effects on OS tumor growth, thus potentially establishing it as a diagnostic target. |
format | Online Article Text |
id | pubmed-7107771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-71077712020-04-03 miR-95 promotes osteosarcoma growth by targeting SCNN1A Geng, Yannan Zhao, Shaorong Jia, Yutao Xia, Gang Li, Huiming Fang, Zhao Zhang, Quan Tian, Rong Oncol Rep Articles Osteosarcoma (OS) is a common malignant bone tumor, presenting particularly in children and young adults, and accounts for approximately 19% of all malignant bone cancers. Despite advances in OS treatment, long-term prognosis remains poor. miRNAs are non-coding single-stranded RNAs ~22 nucleotides in length. Increasing evidence suggests that numerous miRNAs may play critical roles in tumorigenesis and tumor progression; however, the role of miR-95 in OS has not been examined. In the present study, we investigated the role of miR-95 in OS using in vitro and in vivo models and publicly available expression data. Our findings indicate that abnormal miR-95 expression occurs in OS, according to the Gene Expression Omnibus (GEO) database. The miR-95 inhibitor reduced cell proliferation and promoted apoptosis in OS cell lines as detected by EdU staining, TUNEL staining and flow cytometry. Furthermore, a dual luciferase reporter assay revealed that miR-95 regulates the cell cycle of OS cells and apoptosis by targeting sodium channel epithelial 1α subunit (SCNN1A). Additionally, miR-95 antagomir suppressed the growth of U2OS xenograft tumors in a mouse model. In summary, our results suggest that miR-95 induces OS growth in vitro and in vivo by targeting SCNN1A. Our results help clarify the mechanism underlying the miR-95-mediated effects on OS tumor growth, thus potentially establishing it as a diagnostic target. D.A. Spandidos 2020-05 2020-02-24 /pmc/articles/PMC7107771/ /pubmed/32323794 http://dx.doi.org/10.3892/or.2020.7514 Text en Copyright: © Geng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Geng, Yannan Zhao, Shaorong Jia, Yutao Xia, Gang Li, Huiming Fang, Zhao Zhang, Quan Tian, Rong miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title | miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title_full | miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title_fullStr | miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title_full_unstemmed | miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title_short | miR-95 promotes osteosarcoma growth by targeting SCNN1A |
title_sort | mir-95 promotes osteosarcoma growth by targeting scnn1a |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107771/ https://www.ncbi.nlm.nih.gov/pubmed/32323794 http://dx.doi.org/10.3892/or.2020.7514 |
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