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AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway

Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. The biological roles of AREG in the regulation of the epithelial-mesenchymal transition (EMT) in pancreatic cancer remain unclear. To investigate the expression of epidermal growth...

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Autores principales: Wang, Li, Wang, Lili, Zhang, Hui, Lu, Junliang, Zhang, Zhiwen, Wu, Huanwen, Liang, Zhiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107775/
https://www.ncbi.nlm.nih.gov/pubmed/32323797
http://dx.doi.org/10.3892/or.2020.7523
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author Wang, Li
Wang, Lili
Zhang, Hui
Lu, Junliang
Zhang, Zhiwen
Wu, Huanwen
Liang, Zhiyong
author_facet Wang, Li
Wang, Lili
Zhang, Hui
Lu, Junliang
Zhang, Zhiwen
Wu, Huanwen
Liang, Zhiyong
author_sort Wang, Li
collection PubMed
description Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. The biological roles of AREG in the regulation of the epithelial-mesenchymal transition (EMT) in pancreatic cancer remain unclear. To investigate the expression of epidermal growth factor receptor (EGFR) and AREG in pancreatic cancer cell lines, RT-qPCR, western blot analysis, and ELISA were performed. RNAi and exogenous AREG treatment were used to alter AREG expression. Wound-healing and Transwell assays were performed to evaluate cell migration and invasion abilities. Western blot analysis and immunofluorescence staining were utilized to detect the expression of EMT markers. The protein expression of potential key factors involved in EMT, as well as those of the ERK, AKT, STAT3 and NF-κB pathways, were analysed by western blotting. The role of AREG in tumour growth in vivo was further determined using an orthotopic model of pancreatic cancer. Knockdown of AREG inhibited AsPC-1 cell migration and invasion. AREG knockdown upregulated E-cadherin but downregulated vimentin, Snail and Slug expression in AsPC-1 cells. In addition, AREG stimulation increased cell migration, invasion and EMT in PANC-1 cells, and an NF-κB inhibitor decreased AREG-induced cell migration, invasion and EMT in PANC-1 cells. AREG stimulation increased the nuclear accumulation of NF-κB through the EGFR/ERK signalling pathway to induce EMT. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF-κB signalling pathway in pancreatic cancer cells.
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spelling pubmed-71077752020-04-03 AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway Wang, Li Wang, Lili Zhang, Hui Lu, Junliang Zhang, Zhiwen Wu, Huanwen Liang, Zhiyong Oncol Rep Articles Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. The biological roles of AREG in the regulation of the epithelial-mesenchymal transition (EMT) in pancreatic cancer remain unclear. To investigate the expression of epidermal growth factor receptor (EGFR) and AREG in pancreatic cancer cell lines, RT-qPCR, western blot analysis, and ELISA were performed. RNAi and exogenous AREG treatment were used to alter AREG expression. Wound-healing and Transwell assays were performed to evaluate cell migration and invasion abilities. Western blot analysis and immunofluorescence staining were utilized to detect the expression of EMT markers. The protein expression of potential key factors involved in EMT, as well as those of the ERK, AKT, STAT3 and NF-κB pathways, were analysed by western blotting. The role of AREG in tumour growth in vivo was further determined using an orthotopic model of pancreatic cancer. Knockdown of AREG inhibited AsPC-1 cell migration and invasion. AREG knockdown upregulated E-cadherin but downregulated vimentin, Snail and Slug expression in AsPC-1 cells. In addition, AREG stimulation increased cell migration, invasion and EMT in PANC-1 cells, and an NF-κB inhibitor decreased AREG-induced cell migration, invasion and EMT in PANC-1 cells. AREG stimulation increased the nuclear accumulation of NF-κB through the EGFR/ERK signalling pathway to induce EMT. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF-κB signalling pathway in pancreatic cancer cells. D.A. Spandidos 2020-05 2020-02-27 /pmc/articles/PMC7107775/ /pubmed/32323797 http://dx.doi.org/10.3892/or.2020.7523 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Li
Wang, Lili
Zhang, Hui
Lu, Junliang
Zhang, Zhiwen
Wu, Huanwen
Liang, Zhiyong
AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title_full AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title_fullStr AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title_full_unstemmed AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title_short AREG mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF-κB signalling pathway
title_sort areg mediates the epithelial-mesenchymal transition in pancreatic cancer cells via the egfr/erk/nf-κb signalling pathway
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107775/
https://www.ncbi.nlm.nih.gov/pubmed/32323797
http://dx.doi.org/10.3892/or.2020.7523
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