Using multiplex real time PCR in order to streamline a routine diagnostic service

An increasing number of virology laboratories are now utilising in house real time PCR assays as the frontline diagnostic tests. As the number of tests on offer increases the natural progression from this will be to rationalise their service via multiplexing. Since 2003 we have introduced a large nu...

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Detalles Bibliográficos
Autores principales: Gunson, R.N., Bennett, S., Maclean, A., Carman, W.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108215/
https://www.ncbi.nlm.nih.gov/pubmed/18977692
http://dx.doi.org/10.1016/j.jcv.2008.08.020
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author Gunson, R.N.
Bennett, S.
Maclean, A.
Carman, W.F.
author_facet Gunson, R.N.
Bennett, S.
Maclean, A.
Carman, W.F.
author_sort Gunson, R.N.
collection PubMed
description An increasing number of virology laboratories are now utilising in house real time PCR assays as the frontline diagnostic tests. As the number of tests on offer increases the natural progression from this will be to rationalise their service via multiplexing. Since 2003 we have introduced a large number of qualitative and quantitative multiplex real time PCR assays into our routine testing service. This paper describes the development of the multiplex assays, the problems encountered and the resultant benefits to the routine service.
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spelling pubmed-71082152020-03-31 Using multiplex real time PCR in order to streamline a routine diagnostic service Gunson, R.N. Bennett, S. Maclean, A. Carman, W.F. J Clin Virol Article An increasing number of virology laboratories are now utilising in house real time PCR assays as the frontline diagnostic tests. As the number of tests on offer increases the natural progression from this will be to rationalise their service via multiplexing. Since 2003 we have introduced a large number of qualitative and quantitative multiplex real time PCR assays into our routine testing service. This paper describes the development of the multiplex assays, the problems encountered and the resultant benefits to the routine service. Elsevier B.V. 2008-12 2008-11-01 /pmc/articles/PMC7108215/ /pubmed/18977692 http://dx.doi.org/10.1016/j.jcv.2008.08.020 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gunson, R.N.
Bennett, S.
Maclean, A.
Carman, W.F.
Using multiplex real time PCR in order to streamline a routine diagnostic service
title Using multiplex real time PCR in order to streamline a routine diagnostic service
title_full Using multiplex real time PCR in order to streamline a routine diagnostic service
title_fullStr Using multiplex real time PCR in order to streamline a routine diagnostic service
title_full_unstemmed Using multiplex real time PCR in order to streamline a routine diagnostic service
title_short Using multiplex real time PCR in order to streamline a routine diagnostic service
title_sort using multiplex real time pcr in order to streamline a routine diagnostic service
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108215/
https://www.ncbi.nlm.nih.gov/pubmed/18977692
http://dx.doi.org/10.1016/j.jcv.2008.08.020
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