Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay

BACKGROUND: Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups. OBJECTIVE: To evaluate the performance of a rapid antigenic test for all hMPV genotypes. STUDY DESIGN: Frozen nasopharyngeal aspirates from 93 individuals with ARTI...

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Detalles Bibliográficos
Autores principales: Kukavica-Ibrulj, Irena, Boivin, Guy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108374/
https://www.ncbi.nlm.nih.gov/pubmed/19010729
http://dx.doi.org/10.1016/j.jcv.2008.09.011
Descripción
Sumario:BACKGROUND: Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups. OBJECTIVE: To evaluate the performance of a rapid antigenic test for all hMPV genotypes. STUDY DESIGN: Frozen nasopharyngeal aspirates from 93 individuals with ARTI were analyzed for the presence of hMPV antigens using an enzyme immunoassay (EIA, Biotrin Ltd.). RESULTS: The hMPV EIA showed a sensitivity of 81%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 77% compared to viral culture and RT-PCR. CONCLUSION: The Biotrin hMPV EIA is a convenient alternative to cell culture for detection of hMPV with an excellent specificity.

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