Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay

BACKGROUND: Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups. OBJECTIVE: To evaluate the performance of a rapid antigenic test for all hMPV genotypes. STUDY DESIGN: Frozen nasopharyngeal aspirates from 93 individuals with ARTI...

Descripción completa

Detalles Bibliográficos
Autores principales: Kukavica-Ibrulj, Irena, Boivin, Guy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108374/
https://www.ncbi.nlm.nih.gov/pubmed/19010729
http://dx.doi.org/10.1016/j.jcv.2008.09.011
_version_ 1783512798730387456
author Kukavica-Ibrulj, Irena
Boivin, Guy
author_facet Kukavica-Ibrulj, Irena
Boivin, Guy
author_sort Kukavica-Ibrulj, Irena
collection PubMed
description BACKGROUND: Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups. OBJECTIVE: To evaluate the performance of a rapid antigenic test for all hMPV genotypes. STUDY DESIGN: Frozen nasopharyngeal aspirates from 93 individuals with ARTI were analyzed for the presence of hMPV antigens using an enzyme immunoassay (EIA, Biotrin Ltd.). RESULTS: The hMPV EIA showed a sensitivity of 81%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 77% compared to viral culture and RT-PCR. CONCLUSION: The Biotrin hMPV EIA is a convenient alternative to cell culture for detection of hMPV with an excellent specificity.
format Online
Article
Text
id pubmed-7108374
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71083742020-03-31 Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay Kukavica-Ibrulj, Irena Boivin, Guy J Clin Virol Article BACKGROUND: Human metapneumovirus (hMPV) is associated with acute respiratory tract infections (ARTI) in patients from all age groups. OBJECTIVE: To evaluate the performance of a rapid antigenic test for all hMPV genotypes. STUDY DESIGN: Frozen nasopharyngeal aspirates from 93 individuals with ARTI were analyzed for the presence of hMPV antigens using an enzyme immunoassay (EIA, Biotrin Ltd.). RESULTS: The hMPV EIA showed a sensitivity of 81%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 77% compared to viral culture and RT-PCR. CONCLUSION: The Biotrin hMPV EIA is a convenient alternative to cell culture for detection of hMPV with an excellent specificity. Elsevier B.V. 2009-01 2008-11-17 /pmc/articles/PMC7108374/ /pubmed/19010729 http://dx.doi.org/10.1016/j.jcv.2008.09.011 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kukavica-Ibrulj, Irena
Boivin, Guy
Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title_full Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title_fullStr Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title_full_unstemmed Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title_short Detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
title_sort detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108374/
https://www.ncbi.nlm.nih.gov/pubmed/19010729
http://dx.doi.org/10.1016/j.jcv.2008.09.011
work_keys_str_mv AT kukavicaibruljirena detectionofhumanmetapneumovirusantigensinnasopharyngealaspiratesusinganenzymeimmunoassay
AT boivinguy detectionofhumanmetapneumovirusantigensinnasopharyngealaspiratesusinganenzymeimmunoassay

Ejemplares similares