Cargando…

Detection of human coronaviruses in children with acute gastroenteritis

BACKGROUND: Human coronaviruses (HCoVs) are known respiratory pathogens. Moreover, coronavirus-like particles have been seen by electron microscope in stools, and SARS-HCoV has been isolated from intestinal tissue and detected in stool samples. OBJECTIVES: To find out if HCoVs can be found in stools...

Descripción completa

Detalles Bibliográficos
Autores principales: Risku, Minna, Lappalainen, Suvi, Räsänen, Sirpa, Vesikari, Timo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108425/
https://www.ncbi.nlm.nih.gov/pubmed/20233673
http://dx.doi.org/10.1016/j.jcv.2010.02.013
_version_ 1783512809880944640
author Risku, Minna
Lappalainen, Suvi
Räsänen, Sirpa
Vesikari, Timo
author_facet Risku, Minna
Lappalainen, Suvi
Räsänen, Sirpa
Vesikari, Timo
author_sort Risku, Minna
collection PubMed
description BACKGROUND: Human coronaviruses (HCoVs) are known respiratory pathogens. Moreover, coronavirus-like particles have been seen by electron microscope in stools, and SARS-HCoV has been isolated from intestinal tissue and detected in stool samples. OBJECTIVES: To find out if HCoVs can be found in stools of children with acute gastroenteritis and to assess the significance of HCoVs in the etiology of acute gastroenteritis in children. STUDY DESIGN: 878 stool specimens from children with acute gastroenteritis and 112 from control children were tested by RT-PCR to detect HCoV groups 1B, 2A and SARS. HCoVs were typed by sequencing all PCR positive samples. RESULTS: Twenty-two (2.5%) of the 878 stool specimens of children with acute gastroenteritis were positive for HCoVs. The following HCoV types were detected: OC43 (10 cases, 45.5%), HKU1 (6 cases, 27.3%), 229E (2 cases, 9.1%) and NL63 (4 cases, 18.2%). In 4 of the cases a HCoV was the only detected virus; in the remaining cases rotavirus or norovirus was found in the same sample. In control groups there were two HCoV positive samples of 112 tested. CONCLUSIONS: This study shows that all known non-SARS HCoVs can be found in stools of children with acute gastroenteritis. On the basis of this study, the significance of coronaviruses as gastrointestinal pathogens in children appears minor, since most of the coronavirus findings were co-infections with known gastroenteritis viruses.
format Online
Article
Text
id pubmed-7108425
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71084252020-03-31 Detection of human coronaviruses in children with acute gastroenteritis Risku, Minna Lappalainen, Suvi Räsänen, Sirpa Vesikari, Timo J Clin Virol Article BACKGROUND: Human coronaviruses (HCoVs) are known respiratory pathogens. Moreover, coronavirus-like particles have been seen by electron microscope in stools, and SARS-HCoV has been isolated from intestinal tissue and detected in stool samples. OBJECTIVES: To find out if HCoVs can be found in stools of children with acute gastroenteritis and to assess the significance of HCoVs in the etiology of acute gastroenteritis in children. STUDY DESIGN: 878 stool specimens from children with acute gastroenteritis and 112 from control children were tested by RT-PCR to detect HCoV groups 1B, 2A and SARS. HCoVs were typed by sequencing all PCR positive samples. RESULTS: Twenty-two (2.5%) of the 878 stool specimens of children with acute gastroenteritis were positive for HCoVs. The following HCoV types were detected: OC43 (10 cases, 45.5%), HKU1 (6 cases, 27.3%), 229E (2 cases, 9.1%) and NL63 (4 cases, 18.2%). In 4 of the cases a HCoV was the only detected virus; in the remaining cases rotavirus or norovirus was found in the same sample. In control groups there were two HCoV positive samples of 112 tested. CONCLUSIONS: This study shows that all known non-SARS HCoVs can be found in stools of children with acute gastroenteritis. On the basis of this study, the significance of coronaviruses as gastrointestinal pathogens in children appears minor, since most of the coronavirus findings were co-infections with known gastroenteritis viruses. Elsevier B.V. 2010-05 2010-03-15 /pmc/articles/PMC7108425/ /pubmed/20233673 http://dx.doi.org/10.1016/j.jcv.2010.02.013 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Risku, Minna
Lappalainen, Suvi
Räsänen, Sirpa
Vesikari, Timo
Detection of human coronaviruses in children with acute gastroenteritis
title Detection of human coronaviruses in children with acute gastroenteritis
title_full Detection of human coronaviruses in children with acute gastroenteritis
title_fullStr Detection of human coronaviruses in children with acute gastroenteritis
title_full_unstemmed Detection of human coronaviruses in children with acute gastroenteritis
title_short Detection of human coronaviruses in children with acute gastroenteritis
title_sort detection of human coronaviruses in children with acute gastroenteritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108425/
https://www.ncbi.nlm.nih.gov/pubmed/20233673
http://dx.doi.org/10.1016/j.jcv.2010.02.013
work_keys_str_mv AT riskuminna detectionofhumancoronavirusesinchildrenwithacutegastroenteritis
AT lappalainensuvi detectionofhumancoronavirusesinchildrenwithacutegastroenteritis
AT rasanensirpa detectionofhumancoronavirusesinchildrenwithacutegastroenteritis
AT vesikaritimo detectionofhumancoronavirusesinchildrenwithacutegastroenteritis