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Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry

Red bayberry (Morella rubra) is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published. We updated the linkage map of the species by adding 118 SSR markers and the female-specific marker MrFT2_BD-SEX. The integrated map included eight linkage groups a...

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Autores principales: Wang, Yan, Jia, Hui-Min, Shen, Yu-Tong, Zhao, Hai-Bo, Yang, Qin-Song, Zhu, Chang-Qing, Sun, De-Li, Wang, Guo-Yun, Zhou, Chao-Chao, Jiao, Yun, Chai, Chun-Yan, Yan, Li-Ju, Li, Xiong-Wei, Jia, Hui-Juan, Gao, Zhong-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109115/
https://www.ncbi.nlm.nih.gov/pubmed/32257239
http://dx.doi.org/10.1038/s41438-020-0276-6
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author Wang, Yan
Jia, Hui-Min
Shen, Yu-Tong
Zhao, Hai-Bo
Yang, Qin-Song
Zhu, Chang-Qing
Sun, De-Li
Wang, Guo-Yun
Zhou, Chao-Chao
Jiao, Yun
Chai, Chun-Yan
Yan, Li-Ju
Li, Xiong-Wei
Jia, Hui-Juan
Gao, Zhong-Shan
author_facet Wang, Yan
Jia, Hui-Min
Shen, Yu-Tong
Zhao, Hai-Bo
Yang, Qin-Song
Zhu, Chang-Qing
Sun, De-Li
Wang, Guo-Yun
Zhou, Chao-Chao
Jiao, Yun
Chai, Chun-Yan
Yan, Li-Ju
Li, Xiong-Wei
Jia, Hui-Juan
Gao, Zhong-Shan
author_sort Wang, Yan
collection PubMed
description Red bayberry (Morella rubra) is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published. We updated the linkage map of the species by adding 118 SSR markers and the female-specific marker MrFT2_BD-SEX. The integrated map included eight linkage groups and spanned 491 cM. Eleven sex-associated markers were identified, six of which were located in linkage group 8, in agreement with the previously reported location of the sex-determining region. The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions. Among the females of the accessions, we found two female-specific alleles, designated W-b (151 bp) and W-d (129 bp). We previously found that ‘Dongkui’, a female cultivar, could produce viable pollen (we refer to such plants ‘Dongkui-male’) and serve as the paternal parent in crosses. The genotypes of the MrFT2_BD-SEX marker were W-b/Z in ‘Biqi’ and W-d/Z in ‘Dongkui-male’. The progeny of a cross between these parents produced a 3:1 female (W-) to male (ZZ) ratio and the expected 1:1:1:1 ratio of W-b/W-d: W-b/Z: W-d/Z: Z/Z. In addition, the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes. Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus (W) in a small genomic region (59 kb and less than 3.3 cM). Furthermore, we have produced a homozygous “super female” (WW) that should produce all-female offspring in the F2 generation, providing a foundation for commercial use and presenting great potential for use in modern breeding programs.
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spelling pubmed-71091152020-04-06 Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry Wang, Yan Jia, Hui-Min Shen, Yu-Tong Zhao, Hai-Bo Yang, Qin-Song Zhu, Chang-Qing Sun, De-Li Wang, Guo-Yun Zhou, Chao-Chao Jiao, Yun Chai, Chun-Yan Yan, Li-Ju Li, Xiong-Wei Jia, Hui-Juan Gao, Zhong-Shan Hortic Res Article Red bayberry (Morella rubra) is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published. We updated the linkage map of the species by adding 118 SSR markers and the female-specific marker MrFT2_BD-SEX. The integrated map included eight linkage groups and spanned 491 cM. Eleven sex-associated markers were identified, six of which were located in linkage group 8, in agreement with the previously reported location of the sex-determining region. The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions. Among the females of the accessions, we found two female-specific alleles, designated W-b (151 bp) and W-d (129 bp). We previously found that ‘Dongkui’, a female cultivar, could produce viable pollen (we refer to such plants ‘Dongkui-male’) and serve as the paternal parent in crosses. The genotypes of the MrFT2_BD-SEX marker were W-b/Z in ‘Biqi’ and W-d/Z in ‘Dongkui-male’. The progeny of a cross between these parents produced a 3:1 female (W-) to male (ZZ) ratio and the expected 1:1:1:1 ratio of W-b/W-d: W-b/Z: W-d/Z: Z/Z. In addition, the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes. Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus (W) in a small genomic region (59 kb and less than 3.3 cM). Furthermore, we have produced a homozygous “super female” (WW) that should produce all-female offspring in the F2 generation, providing a foundation for commercial use and presenting great potential for use in modern breeding programs. Nature Publishing Group UK 2020-04-01 /pmc/articles/PMC7109115/ /pubmed/32257239 http://dx.doi.org/10.1038/s41438-020-0276-6 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Yan
Jia, Hui-Min
Shen, Yu-Tong
Zhao, Hai-Bo
Yang, Qin-Song
Zhu, Chang-Qing
Sun, De-Li
Wang, Guo-Yun
Zhou, Chao-Chao
Jiao, Yun
Chai, Chun-Yan
Yan, Li-Ju
Li, Xiong-Wei
Jia, Hui-Juan
Gao, Zhong-Shan
Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title_full Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title_fullStr Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title_full_unstemmed Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title_short Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
title_sort construction of an anchoring ssr marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109115/
https://www.ncbi.nlm.nih.gov/pubmed/32257239
http://dx.doi.org/10.1038/s41438-020-0276-6
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