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A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function

Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-te...

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Autores principales: Perry, Christopher J., Warren, Eleanor C., Damstra-Oddy, Joseph L., Storey, Claire, Francione, Lisa M., Annesley, Sarah J., Fisher, Paul R., Müller-Taubenberger, Annette, Williams, Robin S.B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109396/
https://www.ncbi.nlm.nih.gov/pubmed/32258439
http://dx.doi.org/10.1016/j.bbrep.2020.100751
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author Perry, Christopher J.
Warren, Eleanor C.
Damstra-Oddy, Joseph L.
Storey, Claire
Francione, Lisa M.
Annesley, Sarah J.
Fisher, Paul R.
Müller-Taubenberger, Annette
Williams, Robin S.B.
author_facet Perry, Christopher J.
Warren, Eleanor C.
Damstra-Oddy, Joseph L.
Storey, Claire
Francione, Lisa M.
Annesley, Sarah J.
Fisher, Paul R.
Müller-Taubenberger, Annette
Williams, Robin S.B.
author_sort Perry, Christopher J.
collection PubMed
description Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function.
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spelling pubmed-71093962020-04-03 A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function Perry, Christopher J. Warren, Eleanor C. Damstra-Oddy, Joseph L. Storey, Claire Francione, Lisa M. Annesley, Sarah J. Fisher, Paul R. Müller-Taubenberger, Annette Williams, Robin S.B. Biochem Biophys Rep Research Article Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function. Elsevier 2020-03-25 /pmc/articles/PMC7109396/ /pubmed/32258439 http://dx.doi.org/10.1016/j.bbrep.2020.100751 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Perry, Christopher J.
Warren, Eleanor C.
Damstra-Oddy, Joseph L.
Storey, Claire
Francione, Lisa M.
Annesley, Sarah J.
Fisher, Paul R.
Müller-Taubenberger, Annette
Williams, Robin S.B.
A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title_full A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title_fullStr A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title_full_unstemmed A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title_short A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
title_sort dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109396/
https://www.ncbi.nlm.nih.gov/pubmed/32258439
http://dx.doi.org/10.1016/j.bbrep.2020.100751
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