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A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function
Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-te...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109396/ https://www.ncbi.nlm.nih.gov/pubmed/32258439 http://dx.doi.org/10.1016/j.bbrep.2020.100751 |
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author | Perry, Christopher J. Warren, Eleanor C. Damstra-Oddy, Joseph L. Storey, Claire Francione, Lisa M. Annesley, Sarah J. Fisher, Paul R. Müller-Taubenberger, Annette Williams, Robin S.B. |
author_facet | Perry, Christopher J. Warren, Eleanor C. Damstra-Oddy, Joseph L. Storey, Claire Francione, Lisa M. Annesley, Sarah J. Fisher, Paul R. Müller-Taubenberger, Annette Williams, Robin S.B. |
author_sort | Perry, Christopher J. |
collection | PubMed |
description | Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function. |
format | Online Article Text |
id | pubmed-7109396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-71093962020-04-03 A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function Perry, Christopher J. Warren, Eleanor C. Damstra-Oddy, Joseph L. Storey, Claire Francione, Lisa M. Annesley, Sarah J. Fisher, Paul R. Müller-Taubenberger, Annette Williams, Robin S.B. Biochem Biophys Rep Research Article Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function. Elsevier 2020-03-25 /pmc/articles/PMC7109396/ /pubmed/32258439 http://dx.doi.org/10.1016/j.bbrep.2020.100751 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Perry, Christopher J. Warren, Eleanor C. Damstra-Oddy, Joseph L. Storey, Claire Francione, Lisa M. Annesley, Sarah J. Fisher, Paul R. Müller-Taubenberger, Annette Williams, Robin S.B. A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title | A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title_full | A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title_fullStr | A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title_full_unstemmed | A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title_short | A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
title_sort | dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109396/ https://www.ncbi.nlm.nih.gov/pubmed/32258439 http://dx.doi.org/10.1016/j.bbrep.2020.100751 |
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