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Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3

BACKGROUND: The filamentous fungus Trichoderma reesei is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low. RESULTS: In this study,...

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Autores principales: Chen, Yumeng, Wu, Chuan, Fan, Xingjia, Zhao, Xinqing, Zhao, Xihua, Shen, Tao, Wei, Dongzhi, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110754/
https://www.ncbi.nlm.nih.gov/pubmed/32266008
http://dx.doi.org/10.1186/s13068-020-01701-3
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author Chen, Yumeng
Wu, Chuan
Fan, Xingjia
Zhao, Xinqing
Zhao, Xihua
Shen, Tao
Wei, Dongzhi
Wang, Wei
author_facet Chen, Yumeng
Wu, Chuan
Fan, Xingjia
Zhao, Xinqing
Zhao, Xihua
Shen, Tao
Wei, Dongzhi
Wang, Wei
author_sort Chen, Yumeng
collection PubMed
description BACKGROUND: The filamentous fungus Trichoderma reesei is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low. RESULTS: In this study, the truncation of cellulase activator ACE3 was identified and characterized in T. reesei classical mutant NG14 and its direct descendants for the first time. We demonstrated that the truncated ACE3 is the crucial cause of cellulase hyper-production in T. reesei NG14 branch. Replacing the native ACE3 with truncated ACE3 in other T. reesei strains remarkably improves cellulase production. By truncating ACE3, we engineered a T. reesei mutant, PC-3-7-A723, capable of producing more cellulase than other strains. In a 30-L fermenter, fed-batch fermentation with PC-3-7-A723 drastically increased the maximum cellulase titer (FPase) to 102.63 IU/mL at 240 h, which constitutes a 20–30% improvement to that of the parental strain PC-3-7. CONCLUSIONS: This work characterized the function of truncated ACE3 and demonstrated that analysis of classical mutants allows rational engineering of mutant strains with improved cellulase production necessary to process lignocellulosic biomass. Our rational engineering strategy might be useful for enhancing the production of other bio-based products.
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spelling pubmed-71107542020-04-07 Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3 Chen, Yumeng Wu, Chuan Fan, Xingjia Zhao, Xinqing Zhao, Xihua Shen, Tao Wei, Dongzhi Wang, Wei Biotechnol Biofuels Research BACKGROUND: The filamentous fungus Trichoderma reesei is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low. RESULTS: In this study, the truncation of cellulase activator ACE3 was identified and characterized in T. reesei classical mutant NG14 and its direct descendants for the first time. We demonstrated that the truncated ACE3 is the crucial cause of cellulase hyper-production in T. reesei NG14 branch. Replacing the native ACE3 with truncated ACE3 in other T. reesei strains remarkably improves cellulase production. By truncating ACE3, we engineered a T. reesei mutant, PC-3-7-A723, capable of producing more cellulase than other strains. In a 30-L fermenter, fed-batch fermentation with PC-3-7-A723 drastically increased the maximum cellulase titer (FPase) to 102.63 IU/mL at 240 h, which constitutes a 20–30% improvement to that of the parental strain PC-3-7. CONCLUSIONS: This work characterized the function of truncated ACE3 and demonstrated that analysis of classical mutants allows rational engineering of mutant strains with improved cellulase production necessary to process lignocellulosic biomass. Our rational engineering strategy might be useful for enhancing the production of other bio-based products. BioMed Central 2020-04-01 /pmc/articles/PMC7110754/ /pubmed/32266008 http://dx.doi.org/10.1186/s13068-020-01701-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Yumeng
Wu, Chuan
Fan, Xingjia
Zhao, Xinqing
Zhao, Xihua
Shen, Tao
Wei, Dongzhi
Wang, Wei
Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title_full Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title_fullStr Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title_full_unstemmed Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title_short Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3
title_sort engineering of trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ace3
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110754/
https://www.ncbi.nlm.nih.gov/pubmed/32266008
http://dx.doi.org/10.1186/s13068-020-01701-3
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