Cargando…

Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR

INTRODUCTION: Acute lower respiratory tract infection in children causes significant morbidity in the developing countries. Documentation of virus infection using PCR and clinical characteristics of patients affected with viral pneumonia are reviewed in this study. METHODS: 51 children less than thr...

Descripción completa

Detalles Bibliográficos
Autores principales: Meligy, Bassant, Sayed, Amal, Ismail, Dalia Kadry, Kamal, Dina, Abdel-Latif, Walaa, Erfan, Dina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Production and hosting by Elsevier B.V. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110902/
https://www.ncbi.nlm.nih.gov/pubmed/32288487
http://dx.doi.org/10.1016/j.epag.2015.11.005
_version_ 1783513164348915712
author Meligy, Bassant
Sayed, Amal
Ismail, Dalia Kadry
Kamal, Dina
Abdel-Latif, Walaa
Erfan, Dina M.
author_facet Meligy, Bassant
Sayed, Amal
Ismail, Dalia Kadry
Kamal, Dina
Abdel-Latif, Walaa
Erfan, Dina M.
author_sort Meligy, Bassant
collection PubMed
description INTRODUCTION: Acute lower respiratory tract infection in children causes significant morbidity in the developing countries. Documentation of virus infection using PCR and clinical characteristics of patients affected with viral pneumonia are reviewed in this study. METHODS: 51 children less than three years admitted to the Pediatric Hospital, Cairo University with viral pneumonia were included. All patients had undergone nasopharyngeal aspirate for PCR viral detection. RESULTS: A total of 51 cases were enrolled in the study, of which 7 cases were negative while 44 children were positive for viruses. The most common respiratory virus was Rhinovirus in 32 patients (72.2%), then parainfluenza virus (PIV) in 12 (27.3%), of which subtypes PIV1 were 2 (4.5%), PIV3 were 5 (11.4%) and PIV4 were 5 (11.4%) cases. The third common viruses were respiratory syncytial virus (RSV) in 9 (20.5%) cases of which 3 (6.8%) were RSVA and 6 (13.6%) were RSVB and adenovirus in 9 cases (20.5%). Boca virus was found in 8 (18.2%) patients, corona virus 2 (4.5%) patients, H1N1 2 (4.5%) patients, enterovirus 2 patients (4.5%) and human metapneumovirus in one case (2.3%). Influenza B and PIV2 were not detected. Coinfection was found in 28 (63.7%). Mortality occurred in 12 (23.5%). There was no significant relation between virus type or coinfection with disease severity. CONCLUSIONS: RV was the most commonly detected virus in children under 3 years admitted with acute lower respiratory tract infections. Coinfection was present in the majority of our patients; however it was not related significantly to parameters of disease severity.
format Online
Article
Text
id pubmed-7110902
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Authors. Production and hosting by Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71109022020-04-02 Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR Meligy, Bassant Sayed, Amal Ismail, Dalia Kadry Kamal, Dina Abdel-Latif, Walaa Erfan, Dina M. Gaz Egypt Paediatr Assoc Article INTRODUCTION: Acute lower respiratory tract infection in children causes significant morbidity in the developing countries. Documentation of virus infection using PCR and clinical characteristics of patients affected with viral pneumonia are reviewed in this study. METHODS: 51 children less than three years admitted to the Pediatric Hospital, Cairo University with viral pneumonia were included. All patients had undergone nasopharyngeal aspirate for PCR viral detection. RESULTS: A total of 51 cases were enrolled in the study, of which 7 cases were negative while 44 children were positive for viruses. The most common respiratory virus was Rhinovirus in 32 patients (72.2%), then parainfluenza virus (PIV) in 12 (27.3%), of which subtypes PIV1 were 2 (4.5%), PIV3 were 5 (11.4%) and PIV4 were 5 (11.4%) cases. The third common viruses were respiratory syncytial virus (RSV) in 9 (20.5%) cases of which 3 (6.8%) were RSVA and 6 (13.6%) were RSVB and adenovirus in 9 cases (20.5%). Boca virus was found in 8 (18.2%) patients, corona virus 2 (4.5%) patients, H1N1 2 (4.5%) patients, enterovirus 2 patients (4.5%) and human metapneumovirus in one case (2.3%). Influenza B and PIV2 were not detected. Coinfection was found in 28 (63.7%). Mortality occurred in 12 (23.5%). There was no significant relation between virus type or coinfection with disease severity. CONCLUSIONS: RV was the most commonly detected virus in children under 3 years admitted with acute lower respiratory tract infections. Coinfection was present in the majority of our patients; however it was not related significantly to parameters of disease severity. The Authors. Production and hosting by Elsevier B.V. 2016-03 2015-12-30 /pmc/articles/PMC7110902/ /pubmed/32288487 http://dx.doi.org/10.1016/j.epag.2015.11.005 Text en © 2015 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Meligy, Bassant
Sayed, Amal
Ismail, Dalia Kadry
Kamal, Dina
Abdel-Latif, Walaa
Erfan, Dina M.
Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title_full Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title_fullStr Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title_full_unstemmed Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title_short Detection of viral acute lower respiratory tract infection in hospitalized infants using real-time PCR
title_sort detection of viral acute lower respiratory tract infection in hospitalized infants using real-time pcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7110902/
https://www.ncbi.nlm.nih.gov/pubmed/32288487
http://dx.doi.org/10.1016/j.epag.2015.11.005
work_keys_str_mv AT meligybassant detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr
AT sayedamal detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr
AT ismaildaliakadry detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr
AT kamaldina detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr
AT abdellatifwalaa detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr
AT erfandinam detectionofviralacutelowerrespiratorytractinfectioninhospitalizedinfantsusingrealtimepcr