Cargando…
A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate
Since October 2010, severe porcine epidemic diarrhea (PED) outbreaks caused by highly virulent PED virus (PEDV) strains have occurred continuously in the Chinese pig population and caused considerable economic losses. Although PEDV vaccines based on classical PEDV strains have been widely used in Ch...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111334/ https://www.ncbi.nlm.nih.gov/pubmed/30342075 http://dx.doi.org/10.1016/j.virusres.2018.10.012 |
_version_ | 1783513267789889536 |
---|---|
author | Liu, Xinsheng Zhang, Qiaoling Zhang, Liping Zhou, Peng Yang, Jun Fang, Yuzhen Dong, Zhaoliang Zhao, Donghong Li, Weiyan Feng, Jiaxin Cui, Baofeng Zhang, Yongguang Wang, Yonglu |
author_facet | Liu, Xinsheng Zhang, Qiaoling Zhang, Liping Zhou, Peng Yang, Jun Fang, Yuzhen Dong, Zhaoliang Zhao, Donghong Li, Weiyan Feng, Jiaxin Cui, Baofeng Zhang, Yongguang Wang, Yonglu |
author_sort | Liu, Xinsheng |
collection | PubMed |
description | Since October 2010, severe porcine epidemic diarrhea (PED) outbreaks caused by highly virulent PED virus (PEDV) strains have occurred continuously in the Chinese pig population and caused considerable economic losses. Although PEDV vaccines based on classical PEDV strains have been widely used in China in recent years, the morbidity and mortality in piglets remain high. Therefore, virulent genotype GII PEDV strains that are prevalent in the field should be isolated and used to develop next-generation vaccines. In the present study, a Chinese virulent genotype GIIb PEDV strain, CH/HNPJ/2017, was serially propagated in Vero cells for up to 90 passages. The S genes contained typical insertions and deletions that were also found in other recently isolated highly virulent PEDV strains from China and other countries and had two neighboring unique insertion mutations, which resulted in four amino acid changes in the S1 region of passages P10 and P60. Pig infection studies revealed that the CH/HNPJ/2017 strain was highly virulent in piglets, and the median pig diarrhea dose (PDD(50)) was 7.68 log(10)PDD(50)/3 mL. Furthermore, the cell-adapted CH/HNPJ/2017 strain elicited potent serum IgG and neutralizing antibody responses in immunized pigs when it was used as an inactivated vaccine candidate. In addition, the pigs that received the experimental inactivated vaccines were partially protected (3/5) against subsequent viral challenge. In brief, these data indicate that the CH/HNPJ/2017 strain is a promising candidate for developing a safe and effective PEDV vaccine in the future. |
format | Online Article Text |
id | pubmed-7111334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71113342020-04-02 A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate Liu, Xinsheng Zhang, Qiaoling Zhang, Liping Zhou, Peng Yang, Jun Fang, Yuzhen Dong, Zhaoliang Zhao, Donghong Li, Weiyan Feng, Jiaxin Cui, Baofeng Zhang, Yongguang Wang, Yonglu Virus Res Article Since October 2010, severe porcine epidemic diarrhea (PED) outbreaks caused by highly virulent PED virus (PEDV) strains have occurred continuously in the Chinese pig population and caused considerable economic losses. Although PEDV vaccines based on classical PEDV strains have been widely used in China in recent years, the morbidity and mortality in piglets remain high. Therefore, virulent genotype GII PEDV strains that are prevalent in the field should be isolated and used to develop next-generation vaccines. In the present study, a Chinese virulent genotype GIIb PEDV strain, CH/HNPJ/2017, was serially propagated in Vero cells for up to 90 passages. The S genes contained typical insertions and deletions that were also found in other recently isolated highly virulent PEDV strains from China and other countries and had two neighboring unique insertion mutations, which resulted in four amino acid changes in the S1 region of passages P10 and P60. Pig infection studies revealed that the CH/HNPJ/2017 strain was highly virulent in piglets, and the median pig diarrhea dose (PDD(50)) was 7.68 log(10)PDD(50)/3 mL. Furthermore, the cell-adapted CH/HNPJ/2017 strain elicited potent serum IgG and neutralizing antibody responses in immunized pigs when it was used as an inactivated vaccine candidate. In addition, the pigs that received the experimental inactivated vaccines were partially protected (3/5) against subsequent viral challenge. In brief, these data indicate that the CH/HNPJ/2017 strain is a promising candidate for developing a safe and effective PEDV vaccine in the future. Elsevier B.V. 2019-01-02 2018-10-17 /pmc/articles/PMC7111334/ /pubmed/30342075 http://dx.doi.org/10.1016/j.virusres.2018.10.012 Text en © 2018 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Liu, Xinsheng Zhang, Qiaoling Zhang, Liping Zhou, Peng Yang, Jun Fang, Yuzhen Dong, Zhaoliang Zhao, Donghong Li, Weiyan Feng, Jiaxin Cui, Baofeng Zhang, Yongguang Wang, Yonglu A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title | A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title_full | A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title_fullStr | A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title_full_unstemmed | A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title_short | A newly isolated Chinese virulent genotype GIIb porcine epidemic diarrhea virus strain: Biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
title_sort | newly isolated chinese virulent genotype giib porcine epidemic diarrhea virus strain: biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111334/ https://www.ncbi.nlm.nih.gov/pubmed/30342075 http://dx.doi.org/10.1016/j.virusres.2018.10.012 |
work_keys_str_mv | AT liuxinsheng anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangqiaoling anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangliping anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhoupeng anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT yangjun anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT fangyuzhen anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT dongzhaoliang anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhaodonghong anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT liweiyan anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT fengjiaxin anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT cuibaofeng anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangyongguang anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT wangyonglu anewlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT liuxinsheng newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangqiaoling newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangliping newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhoupeng newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT yangjun newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT fangyuzhen newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT dongzhaoliang newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhaodonghong newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT liweiyan newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT fengjiaxin newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT cuibaofeng newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT zhangyongguang newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate AT wangyonglu newlyisolatedchinesevirulentgenotypegiibporcineepidemicdiarrheavirusstrainbiologicalcharacteristicspathogenicityandimmuneprotectiveeffectsasaninactivatedvaccinecandidate |