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Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application
Lipid has been widely studied as a vehicle and loading vector, but there have been no reports of any such related application in the dental field. The purpose of this research was to fabricate and characterize a nano-size calcium-phosphate lipid (CL) system as a potential vehicle in dental regenerat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111464/ https://www.ncbi.nlm.nih.gov/pubmed/32269987 http://dx.doi.org/10.3389/fchem.2020.00161 |
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author | Zhu, Ningxin Wang, Dan Xie, Fei Qin, Man Lin, Zhiqiang Wang, Yuanyuan |
author_facet | Zhu, Ningxin Wang, Dan Xie, Fei Qin, Man Lin, Zhiqiang Wang, Yuanyuan |
author_sort | Zhu, Ningxin |
collection | PubMed |
description | Lipid has been widely studied as a vehicle and loading vector, but there have been no reports of any such related application in the dental field. The purpose of this research was to fabricate and characterize a nano-size calcium-phosphate lipid (CL) system as a potential vehicle in dental regeneration study, wherein the biocompatibility with dental pulp stem cells (DPSCs) was evaluated. The effect of CL on DPSCs proliferation was analyzed by a CCK-8 assay, and the anti-inflammatory effect was investigated by quantitative polymerase chain reaction (qPCR). Moreover, the effect of CL on odontogenic differentiation of inflamed DPSCs (iDPSCs) was studied by Alizarin red staining, tissue-non-specific alkaline phosphatase (TNAP) staining, qPCR, and western blot analyses. The results of this study showed that CL did not affect the proliferation of DPSCs, it down-regulated the inflammatory-associated markers (IL-1β, IL-6, TNF-α, COX-2) of DPSCs treated with Escherichia coli lipopolysaccharide (LPS), and enhanced the in-vitro odontogenic differentiation potential of iDPSCs. This novel biomaterial has a broad application prospect for its bioactivity and flexible physical property, and thus represents a promising pulpal regeneration material. |
format | Online Article Text |
id | pubmed-7111464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71114642020-04-08 Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application Zhu, Ningxin Wang, Dan Xie, Fei Qin, Man Lin, Zhiqiang Wang, Yuanyuan Front Chem Chemistry Lipid has been widely studied as a vehicle and loading vector, but there have been no reports of any such related application in the dental field. The purpose of this research was to fabricate and characterize a nano-size calcium-phosphate lipid (CL) system as a potential vehicle in dental regeneration study, wherein the biocompatibility with dental pulp stem cells (DPSCs) was evaluated. The effect of CL on DPSCs proliferation was analyzed by a CCK-8 assay, and the anti-inflammatory effect was investigated by quantitative polymerase chain reaction (qPCR). Moreover, the effect of CL on odontogenic differentiation of inflamed DPSCs (iDPSCs) was studied by Alizarin red staining, tissue-non-specific alkaline phosphatase (TNAP) staining, qPCR, and western blot analyses. The results of this study showed that CL did not affect the proliferation of DPSCs, it down-regulated the inflammatory-associated markers (IL-1β, IL-6, TNF-α, COX-2) of DPSCs treated with Escherichia coli lipopolysaccharide (LPS), and enhanced the in-vitro odontogenic differentiation potential of iDPSCs. This novel biomaterial has a broad application prospect for its bioactivity and flexible physical property, and thus represents a promising pulpal regeneration material. Frontiers Media S.A. 2020-03-25 /pmc/articles/PMC7111464/ /pubmed/32269987 http://dx.doi.org/10.3389/fchem.2020.00161 Text en Copyright © 2020 Zhu, Wang, Xie, Qin, Lin and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Zhu, Ningxin Wang, Dan Xie, Fei Qin, Man Lin, Zhiqiang Wang, Yuanyuan Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title | Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title_full | Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title_fullStr | Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title_full_unstemmed | Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title_short | Fabrication and Characterization of Calcium-Phosphate Lipid System for Potential Dental Application |
title_sort | fabrication and characterization of calcium-phosphate lipid system for potential dental application |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111464/ https://www.ncbi.nlm.nih.gov/pubmed/32269987 http://dx.doi.org/10.3389/fchem.2020.00161 |
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