Virus purification and enrichment by hydroxyapatite chromatography on a chip

The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clin...

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Detalles Bibliográficos
Autores principales: Niimi, Miyako, Masuda, Taisuke, Kaihatsu, Kunihiro, Kato, Nobuo, Nakamura, Shota, Nakaya, Takaaki, Arai, Fumihito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111472/
https://www.ncbi.nlm.nih.gov/pubmed/32288247
http://dx.doi.org/10.1016/j.snb.2014.04.011
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author Niimi, Miyako
Masuda, Taisuke
Kaihatsu, Kunihiro
Kato, Nobuo
Nakamura, Shota
Nakaya, Takaaki
Arai, Fumihito
author_facet Niimi, Miyako
Masuda, Taisuke
Kaihatsu, Kunihiro
Kato, Nobuo
Nakamura, Shota
Nakaya, Takaaki
Arai, Fumihito
author_sort Niimi, Miyako
collection PubMed
description The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite particles packed in a micro-column. We demonstrated the purification of virus from a mixture of virus and FBS protein, and enrichment of the virus using this novel microfluidic chip.
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spelling pubmed-71114722020-04-02 Virus purification and enrichment by hydroxyapatite chromatography on a chip Niimi, Miyako Masuda, Taisuke Kaihatsu, Kunihiro Kato, Nobuo Nakamura, Shota Nakaya, Takaaki Arai, Fumihito Sens Actuators B Chem Article The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite particles packed in a micro-column. We demonstrated the purification of virus from a mixture of virus and FBS protein, and enrichment of the virus using this novel microfluidic chip. Elsevier B.V. 2014-10-01 2014-05-06 /pmc/articles/PMC7111472/ /pubmed/32288247 http://dx.doi.org/10.1016/j.snb.2014.04.011 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Niimi, Miyako
Masuda, Taisuke
Kaihatsu, Kunihiro
Kato, Nobuo
Nakamura, Shota
Nakaya, Takaaki
Arai, Fumihito
Virus purification and enrichment by hydroxyapatite chromatography on a chip
title Virus purification and enrichment by hydroxyapatite chromatography on a chip
title_full Virus purification and enrichment by hydroxyapatite chromatography on a chip
title_fullStr Virus purification and enrichment by hydroxyapatite chromatography on a chip
title_full_unstemmed Virus purification and enrichment by hydroxyapatite chromatography on a chip
title_short Virus purification and enrichment by hydroxyapatite chromatography on a chip
title_sort virus purification and enrichment by hydroxyapatite chromatography on a chip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111472/
https://www.ncbi.nlm.nih.gov/pubmed/32288247
http://dx.doi.org/10.1016/j.snb.2014.04.011
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