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Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway

Myricetin, a common dietary flavonoid, was reported to possess many different biological activities such as anti-oxidant, anti-inflammatory, and antiviral effects. In this study, we explored the anti-HSV effects and mechanisms of myricetin both in vitro and in vivo. The results showed that myricetin...

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Autores principales: Li, Wenmiao, Xu, Cuijing, Hao, Cui, Zhang, Yang, Wang, Zhaoqi, Wang, Shuyao, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111628/
https://www.ncbi.nlm.nih.gov/pubmed/32165083
http://dx.doi.org/10.1016/j.antiviral.2020.104714
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author Li, Wenmiao
Xu, Cuijing
Hao, Cui
Zhang, Yang
Wang, Zhaoqi
Wang, Shuyao
Wang, Wei
author_facet Li, Wenmiao
Xu, Cuijing
Hao, Cui
Zhang, Yang
Wang, Zhaoqi
Wang, Shuyao
Wang, Wei
author_sort Li, Wenmiao
collection PubMed
description Myricetin, a common dietary flavonoid, was reported to possess many different biological activities such as anti-oxidant, anti-inflammatory, and antiviral effects. In this study, we explored the anti-HSV effects and mechanisms of myricetin both in vitro and in vivo. The results showed that myricetin possessed anti-HSV-1 and HSV-2 activities with very low toxicity, superior to the effects of acyclovir. Myricetin may block HSV infection through direct interaction with virus gD protein to interfere with virus adsorption and membrane fusion, which was different from the nucleoside analogues such as acyclovir. Myricetin also down-regulate the cellular EGFR/PI3K/Akt signaling pathway to further inhibit HSV infection and its subsequent replication. Most importantly, intraperitoneal therapy of myricetin markedly improved mice survival and reduced virus titers in both lungs and spinal cord. Therefore, the natural dietary flavonoid myricetin has potential to be developed into a novel anti-HSV agent targeting both virus gD protein and cellular EGFR/PI3K/Akt pathway.
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spelling pubmed-71116282020-04-02 Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway Li, Wenmiao Xu, Cuijing Hao, Cui Zhang, Yang Wang, Zhaoqi Wang, Shuyao Wang, Wei Antiviral Res Article Myricetin, a common dietary flavonoid, was reported to possess many different biological activities such as anti-oxidant, anti-inflammatory, and antiviral effects. In this study, we explored the anti-HSV effects and mechanisms of myricetin both in vitro and in vivo. The results showed that myricetin possessed anti-HSV-1 and HSV-2 activities with very low toxicity, superior to the effects of acyclovir. Myricetin may block HSV infection through direct interaction with virus gD protein to interfere with virus adsorption and membrane fusion, which was different from the nucleoside analogues such as acyclovir. Myricetin also down-regulate the cellular EGFR/PI3K/Akt signaling pathway to further inhibit HSV infection and its subsequent replication. Most importantly, intraperitoneal therapy of myricetin markedly improved mice survival and reduced virus titers in both lungs and spinal cord. Therefore, the natural dietary flavonoid myricetin has potential to be developed into a novel anti-HSV agent targeting both virus gD protein and cellular EGFR/PI3K/Akt pathway. Elsevier B.V. 2020-05 2020-03-09 /pmc/articles/PMC7111628/ /pubmed/32165083 http://dx.doi.org/10.1016/j.antiviral.2020.104714 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Li, Wenmiao
Xu, Cuijing
Hao, Cui
Zhang, Yang
Wang, Zhaoqi
Wang, Shuyao
Wang, Wei
Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title_full Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title_fullStr Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title_full_unstemmed Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title_short Inhibition of herpes simplex virus by myricetin through targeting viral gD protein and cellular EGFR/PI3K/Akt pathway
title_sort inhibition of herpes simplex virus by myricetin through targeting viral gd protein and cellular egfr/pi3k/akt pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111628/
https://www.ncbi.nlm.nih.gov/pubmed/32165083
http://dx.doi.org/10.1016/j.antiviral.2020.104714
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