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Detection of non-primate hepaciviruses in UK dogs
Non-primate hepacivirus (NPHV) has been identified in dogs, horses, bats and wild rodents. The presence of NPHV in dogs outside of the USA however is yet to be established. Here we describe for the first time the detection of NPHV in the UK dog population (described throughout the manuscript as CnNP...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111718/ https://www.ncbi.nlm.nih.gov/pubmed/26086431 http://dx.doi.org/10.1016/j.virol.2015.05.005 |
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author | El-Attar, L.M.R. Mitchell, J.A. Brooks Brownlie, H. Priestnall, S.L. Brownlie, J. |
author_facet | El-Attar, L.M.R. Mitchell, J.A. Brooks Brownlie, H. Priestnall, S.L. Brownlie, J. |
author_sort | El-Attar, L.M.R. |
collection | PubMed |
description | Non-primate hepacivirus (NPHV) has been identified in dogs, horses, bats and wild rodents. The presence of NPHV in dogs outside of the USA however is yet to be established. Here we describe for the first time the detection of NPHV in the UK dog population (described throughout the manuscript as CnNPHV). We examined tissues collected from dogs housed in a rehoming kennel where respiratory disease was endemic. CnNPHV RNA was detected in the tracheal tissues of 48/210 dogs by RT-PCR, and in the liver, lung and/or tracheal tissues of 12/20 dogs. The presence of CnNPHV RNA, and its tropism was confirmed by in situ hybridisation. Histopathological examination demonstrated a trend toward higher histopathological scores in CnNPHV RNA positive respiratory tissues, although, this was not statistically significant. Our findings broaden the geographic distribution and our understanding of CnNPHV. Further evidence of CnNPHV replication in canids warrants investigation. |
format | Online Article Text |
id | pubmed-7111718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71117182020-04-02 Detection of non-primate hepaciviruses in UK dogs El-Attar, L.M.R. Mitchell, J.A. Brooks Brownlie, H. Priestnall, S.L. Brownlie, J. Virology Article Non-primate hepacivirus (NPHV) has been identified in dogs, horses, bats and wild rodents. The presence of NPHV in dogs outside of the USA however is yet to be established. Here we describe for the first time the detection of NPHV in the UK dog population (described throughout the manuscript as CnNPHV). We examined tissues collected from dogs housed in a rehoming kennel where respiratory disease was endemic. CnNPHV RNA was detected in the tracheal tissues of 48/210 dogs by RT-PCR, and in the liver, lung and/or tracheal tissues of 12/20 dogs. The presence of CnNPHV RNA, and its tropism was confirmed by in situ hybridisation. Histopathological examination demonstrated a trend toward higher histopathological scores in CnNPHV RNA positive respiratory tissues, although, this was not statistically significant. Our findings broaden the geographic distribution and our understanding of CnNPHV. Further evidence of CnNPHV replication in canids warrants investigation. Published by Elsevier Inc. 2015-10 2015-06-15 /pmc/articles/PMC7111718/ /pubmed/26086431 http://dx.doi.org/10.1016/j.virol.2015.05.005 Text en Copyright © 2015 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article El-Attar, L.M.R. Mitchell, J.A. Brooks Brownlie, H. Priestnall, S.L. Brownlie, J. Detection of non-primate hepaciviruses in UK dogs |
title | Detection of non-primate hepaciviruses in UK dogs |
title_full | Detection of non-primate hepaciviruses in UK dogs |
title_fullStr | Detection of non-primate hepaciviruses in UK dogs |
title_full_unstemmed | Detection of non-primate hepaciviruses in UK dogs |
title_short | Detection of non-primate hepaciviruses in UK dogs |
title_sort | detection of non-primate hepaciviruses in uk dogs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111718/ https://www.ncbi.nlm.nih.gov/pubmed/26086431 http://dx.doi.org/10.1016/j.virol.2015.05.005 |
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