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Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strat...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111734/ https://www.ncbi.nlm.nih.gov/pubmed/25683113 http://dx.doi.org/10.1016/j.rvsc.2015.01.015 |
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author | Shi, Xibao Chang, Yongzhe Zhang, Xiaozhuan Wang, Li Li, Chunxi Jiang, Kai Chen, Jing Wang, Chao Deng, Ruiguang Fan, Jianming Zhang, Gaiping |
author_facet | Shi, Xibao Chang, Yongzhe Zhang, Xiaozhuan Wang, Li Li, Chunxi Jiang, Kai Chen, Jing Wang, Chao Deng, Ruiguang Fan, Jianming Zhang, Gaiping |
author_sort | Shi, Xibao |
collection | PubMed |
description | Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future. |
format | Online Article Text |
id | pubmed-7111734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71117342020-04-02 Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells Shi, Xibao Chang, Yongzhe Zhang, Xiaozhuan Wang, Li Li, Chunxi Jiang, Kai Chen, Jing Wang, Chao Deng, Ruiguang Fan, Jianming Zhang, Gaiping Res Vet Sci Article Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future. Elsevier Ltd. 2015-04 2015-01-30 /pmc/articles/PMC7111734/ /pubmed/25683113 http://dx.doi.org/10.1016/j.rvsc.2015.01.015 Text en Copyright © 2015 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Shi, Xibao Chang, Yongzhe Zhang, Xiaozhuan Wang, Li Li, Chunxi Jiang, Kai Chen, Jing Wang, Chao Deng, Ruiguang Fan, Jianming Zhang, Gaiping Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title | Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title_full | Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title_fullStr | Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title_full_unstemmed | Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title_short | Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells |
title_sort | small interfering rna targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (prrsv) can reduce the replication of prrsv in marc-145 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111734/ https://www.ncbi.nlm.nih.gov/pubmed/25683113 http://dx.doi.org/10.1016/j.rvsc.2015.01.015 |
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