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Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strat...

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Autores principales: Shi, Xibao, Chang, Yongzhe, Zhang, Xiaozhuan, Wang, Li, Li, Chunxi, Jiang, Kai, Chen, Jing, Wang, Chao, Deng, Ruiguang, Fan, Jianming, Zhang, Gaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111734/
https://www.ncbi.nlm.nih.gov/pubmed/25683113
http://dx.doi.org/10.1016/j.rvsc.2015.01.015
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author Shi, Xibao
Chang, Yongzhe
Zhang, Xiaozhuan
Wang, Li
Li, Chunxi
Jiang, Kai
Chen, Jing
Wang, Chao
Deng, Ruiguang
Fan, Jianming
Zhang, Gaiping
author_facet Shi, Xibao
Chang, Yongzhe
Zhang, Xiaozhuan
Wang, Li
Li, Chunxi
Jiang, Kai
Chen, Jing
Wang, Chao
Deng, Ruiguang
Fan, Jianming
Zhang, Gaiping
author_sort Shi, Xibao
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future.
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spelling pubmed-71117342020-04-02 Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells Shi, Xibao Chang, Yongzhe Zhang, Xiaozhuan Wang, Li Li, Chunxi Jiang, Kai Chen, Jing Wang, Chao Deng, Ruiguang Fan, Jianming Zhang, Gaiping Res Vet Sci Article Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future. Elsevier Ltd. 2015-04 2015-01-30 /pmc/articles/PMC7111734/ /pubmed/25683113 http://dx.doi.org/10.1016/j.rvsc.2015.01.015 Text en Copyright © 2015 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Shi, Xibao
Chang, Yongzhe
Zhang, Xiaozhuan
Wang, Li
Li, Chunxi
Jiang, Kai
Chen, Jing
Wang, Chao
Deng, Ruiguang
Fan, Jianming
Zhang, Gaiping
Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title_full Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title_fullStr Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title_full_unstemmed Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title_short Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells
title_sort small interfering rna targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (prrsv) can reduce the replication of prrsv in marc-145 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111734/
https://www.ncbi.nlm.nih.gov/pubmed/25683113
http://dx.doi.org/10.1016/j.rvsc.2015.01.015
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