Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein

Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418 bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19– 367 aa was expres...

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Detalles Bibliográficos
Autores principales: Guo, Hongyan, Guo, Aizhen, Wang, Chong, Yan, Bangfen, Lu, Haisong, Chen, Huanchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111849/
https://www.ncbi.nlm.nih.gov/pubmed/17658563
http://dx.doi.org/10.1016/j.rvsc.2007.05.011
Descripción
Sumario:Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418 bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19– 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did.

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