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Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China

Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) are epizootic swine viruses. To detect and study the evolution of PEDV and PDCoV in central China (Shanxi, Henan, Hubei province), 70 clinical intestinal and fecal samples from piglets with severe watery diarrhea during Augu...

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Autores principales: Li, Dongliang, Feng, Hua, Liu, Yunchao, Chen, Yumei, Wei, Qiang, Wang, Juan, Liu, Dongmin, Huang, Huimin, Su, Yunfang, Wang, Dongyu, Cui, Yinglei, Zhang, Gaiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111851/
https://www.ncbi.nlm.nih.gov/pubmed/30265872
http://dx.doi.org/10.1016/j.rvsc.2018.06.001
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author Li, Dongliang
Feng, Hua
Liu, Yunchao
Chen, Yumei
Wei, Qiang
Wang, Juan
Liu, Dongmin
Huang, Huimin
Su, Yunfang
Wang, Dongyu
Cui, Yinglei
Zhang, Gaiping
author_facet Li, Dongliang
Feng, Hua
Liu, Yunchao
Chen, Yumei
Wei, Qiang
Wang, Juan
Liu, Dongmin
Huang, Huimin
Su, Yunfang
Wang, Dongyu
Cui, Yinglei
Zhang, Gaiping
author_sort Li, Dongliang
collection PubMed
description Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) are epizootic swine viruses. To detect and study the evolution of PEDV and PDCoV in central China (Shanxi, Henan, Hubei province), 70 clinical intestinal and fecal samples from piglets with severe watery diarrhea during August 2015 and June 2016 were collected, tested and analyzed. PEDV was more frequently detected by PCR than PDCoV. Phylogenetic analysis of S genes showed that the 10 PEDV strains from this study clustered into G2a (n = 7) and G2b (n = 3) groups. Additionally, the three G2b strains (PEDV S2△) contained the same specific 3 nt deletion in S2 as other reference strains in G2b. Interestingly, complete genome analysis indicated that CH/hubei/2016 was closer to the US INDEL strain and G2a group. CH/hubei/2016 had one recombination event in S2 gene which may have resulted from AH2012-12 (from G2b group) and CH-ZMDZY-11 (from G2a group). Furthermore, 10 purifying selection sites in S gene indicated an adaptive evolution of PEDV in central China swine herds. These results suggested that Pandemic G2a and G2b are predominant PEDV genotype circulating in central China. In addition, the deletion and recombination identified in S gene suggested PEDV strains of central exhibited an evolutionary variety. However, whether these changes affect the pathogenicity and antigenicity of wild PEDV is unknown and is worth for further investigation.
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spelling pubmed-71118512020-04-02 Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China Li, Dongliang Feng, Hua Liu, Yunchao Chen, Yumei Wei, Qiang Wang, Juan Liu, Dongmin Huang, Huimin Su, Yunfang Wang, Dongyu Cui, Yinglei Zhang, Gaiping Res Vet Sci Article Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) are epizootic swine viruses. To detect and study the evolution of PEDV and PDCoV in central China (Shanxi, Henan, Hubei province), 70 clinical intestinal and fecal samples from piglets with severe watery diarrhea during August 2015 and June 2016 were collected, tested and analyzed. PEDV was more frequently detected by PCR than PDCoV. Phylogenetic analysis of S genes showed that the 10 PEDV strains from this study clustered into G2a (n = 7) and G2b (n = 3) groups. Additionally, the three G2b strains (PEDV S2△) contained the same specific 3 nt deletion in S2 as other reference strains in G2b. Interestingly, complete genome analysis indicated that CH/hubei/2016 was closer to the US INDEL strain and G2a group. CH/hubei/2016 had one recombination event in S2 gene which may have resulted from AH2012-12 (from G2b group) and CH-ZMDZY-11 (from G2a group). Furthermore, 10 purifying selection sites in S gene indicated an adaptive evolution of PEDV in central China swine herds. These results suggested that Pandemic G2a and G2b are predominant PEDV genotype circulating in central China. In addition, the deletion and recombination identified in S gene suggested PEDV strains of central exhibited an evolutionary variety. However, whether these changes affect the pathogenicity and antigenicity of wild PEDV is unknown and is worth for further investigation. Published by Elsevier Ltd. 2018-10 2018-06-13 /pmc/articles/PMC7111851/ /pubmed/30265872 http://dx.doi.org/10.1016/j.rvsc.2018.06.001 Text en © 2018 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Li, Dongliang
Feng, Hua
Liu, Yunchao
Chen, Yumei
Wei, Qiang
Wang, Juan
Liu, Dongmin
Huang, Huimin
Su, Yunfang
Wang, Dongyu
Cui, Yinglei
Zhang, Gaiping
Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title_full Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title_fullStr Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title_full_unstemmed Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title_short Molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in Central China
title_sort molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in central china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111851/
https://www.ncbi.nlm.nih.gov/pubmed/30265872
http://dx.doi.org/10.1016/j.rvsc.2018.06.001
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