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Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus
Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELI...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111879/ https://www.ncbi.nlm.nih.gov/pubmed/19501378 http://dx.doi.org/10.1016/j.rvsc.2009.05.009 |
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author | Sozzi, Enrica Luppi, Andrea Lelli, Davide Martin, Ana Moreno Canelli, Elena Brocchi, Emiliana Lavazza, Antonio Cordioli, Paolo |
author_facet | Sozzi, Enrica Luppi, Andrea Lelli, Davide Martin, Ana Moreno Canelli, Elena Brocchi, Emiliana Lavazza, Antonio Cordioli, Paolo |
author_sort | Sozzi, Enrica |
collection | PubMed |
description | Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELISA was compared with RT-PCR in the examination of 506 specimens collected during 2006–2007 from pigs originating from different farms located in the Po valley. Both faecal samples obtained directly from the rectum of live animals showing clinical signs and intestinal samples collected from the caecum of deceased pigs were included in the study. The correlation between the two methods was higher when testing faecal samples (K = 0.97, 95% CI: 0.94–1.00) than testing intestinal samples (K = 0.62, 95% CI: 0.35–0.89). The use of ELISA technology provided an efficient and effective mean of evaluating the presence of coronavirus PED antigen in field samples and indicates that this procedure is a very useful tool in epidemiological studies. |
format | Online Article Text |
id | pubmed-7111879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71118792020-04-02 Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus Sozzi, Enrica Luppi, Andrea Lelli, Davide Martin, Ana Moreno Canelli, Elena Brocchi, Emiliana Lavazza, Antonio Cordioli, Paolo Res Vet Sci Article Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELISA was compared with RT-PCR in the examination of 506 specimens collected during 2006–2007 from pigs originating from different farms located in the Po valley. Both faecal samples obtained directly from the rectum of live animals showing clinical signs and intestinal samples collected from the caecum of deceased pigs were included in the study. The correlation between the two methods was higher when testing faecal samples (K = 0.97, 95% CI: 0.94–1.00) than testing intestinal samples (K = 0.62, 95% CI: 0.35–0.89). The use of ELISA technology provided an efficient and effective mean of evaluating the presence of coronavirus PED antigen in field samples and indicates that this procedure is a very useful tool in epidemiological studies. Elsevier Ltd. 2010-02 2009-06-05 /pmc/articles/PMC7111879/ /pubmed/19501378 http://dx.doi.org/10.1016/j.rvsc.2009.05.009 Text en Copyright © 2009 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Sozzi, Enrica Luppi, Andrea Lelli, Davide Martin, Ana Moreno Canelli, Elena Brocchi, Emiliana Lavazza, Antonio Cordioli, Paolo Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title | Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title_full | Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title_fullStr | Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title_full_unstemmed | Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title_short | Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus |
title_sort | comparison of enzyme-linked immunosorbent assay and rt-pcr for the detection of porcine epidemic diarrhoea virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111879/ https://www.ncbi.nlm.nih.gov/pubmed/19501378 http://dx.doi.org/10.1016/j.rvsc.2009.05.009 |
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