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Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells

In this study, we confirmed the autophagy induced by porcine reproductive and respiratory syndrome virus (PRRSV) in permissive cells and investigated the role of autophagy in the replication of PRRSV. We first demonstrated that PRRSV infection significantly results in the increased double-membrane v...

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Autores principales: Liu, Qinghao, Qin, Yixian, Zhou, Lei, Kou, Qiuwen, Guo, Xin, Ge, Xinna, Yang, Hanchun, Hu, Hongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111961/
https://www.ncbi.nlm.nih.gov/pubmed/22564420
http://dx.doi.org/10.1016/j.virol.2012.03.022
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author Liu, Qinghao
Qin, Yixian
Zhou, Lei
Kou, Qiuwen
Guo, Xin
Ge, Xinna
Yang, Hanchun
Hu, Hongbo
author_facet Liu, Qinghao
Qin, Yixian
Zhou, Lei
Kou, Qiuwen
Guo, Xin
Ge, Xinna
Yang, Hanchun
Hu, Hongbo
author_sort Liu, Qinghao
collection PubMed
description In this study, we confirmed the autophagy induced by porcine reproductive and respiratory syndrome virus (PRRSV) in permissive cells and investigated the role of autophagy in the replication of PRRSV. We first demonstrated that PRRSV infection significantly results in the increased double-membrane vesicles, the accumulation of LC3 fluorescence puncta, and the raised ratio of LC3-II/β-actin, in MARC-145 cells. Then we discovered that induction of autophagy by rapamycin significantly enhances the viral titers of PRRSV, while inhibition of autophagy by 3-MA and silencing of LC3 gene by siRNA reduces the yield of PRRSV. The results showed functional autolysosomes can be formed after PRRSV infection and the autophagosome–lysosome-fusion inhibitor decreases the virus titers. We also examined the induction of autophagy by PRRSV infection in pulmonary alveolar macrophages. These findings indicate that autophagy induced by PRRSV infection plays a role in sustaining the replication of PRRSV in host cells.
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spelling pubmed-71119612020-04-02 Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells Liu, Qinghao Qin, Yixian Zhou, Lei Kou, Qiuwen Guo, Xin Ge, Xinna Yang, Hanchun Hu, Hongbo Virology Article In this study, we confirmed the autophagy induced by porcine reproductive and respiratory syndrome virus (PRRSV) in permissive cells and investigated the role of autophagy in the replication of PRRSV. We first demonstrated that PRRSV infection significantly results in the increased double-membrane vesicles, the accumulation of LC3 fluorescence puncta, and the raised ratio of LC3-II/β-actin, in MARC-145 cells. Then we discovered that induction of autophagy by rapamycin significantly enhances the viral titers of PRRSV, while inhibition of autophagy by 3-MA and silencing of LC3 gene by siRNA reduces the yield of PRRSV. The results showed functional autolysosomes can be formed after PRRSV infection and the autophagosome–lysosome-fusion inhibitor decreases the virus titers. We also examined the induction of autophagy by PRRSV infection in pulmonary alveolar macrophages. These findings indicate that autophagy induced by PRRSV infection plays a role in sustaining the replication of PRRSV in host cells. Elsevier Inc. 2012-08-01 2012-05-05 /pmc/articles/PMC7111961/ /pubmed/22564420 http://dx.doi.org/10.1016/j.virol.2012.03.022 Text en Copyright © 2012 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Liu, Qinghao
Qin, Yixian
Zhou, Lei
Kou, Qiuwen
Guo, Xin
Ge, Xinna
Yang, Hanchun
Hu, Hongbo
Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title_full Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title_fullStr Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title_full_unstemmed Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title_short Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
title_sort autophagy sustains the replication of porcine reproductive and respiratory virus in host cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111961/
https://www.ncbi.nlm.nih.gov/pubmed/22564420
http://dx.doi.org/10.1016/j.virol.2012.03.022
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