Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways

We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negativ...

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Autores principales: Hasebe, Rie, Sasaki, Michihito, Sawa, Hirofumi, Wada, Ryuichi, Umemura, Takashi, Kimura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111996/
https://www.ncbi.nlm.nih.gov/pubmed/19720389
http://dx.doi.org/10.1016/j.virol.2009.07.032
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author Hasebe, Rie
Sasaki, Michihito
Sawa, Hirofumi
Wada, Ryuichi
Umemura, Takashi
Kimura, Takashi
author_facet Hasebe, Rie
Sasaki, Michihito
Sawa, Hirofumi
Wada, Ryuichi
Umemura, Takashi
Kimura, Takashi
author_sort Hasebe, Rie
collection PubMed
description We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negative form of equine caveolin-1 (ecavY14F), suggesting that EHV-1 enters EBMECs via caveolar endocytosis. EHV-1 entry into E. Derm cells was significantly reduced by ATP depletion and treatments with lysosomotropic agents. Enveloped virions were detected from E. Derm cells by infectious virus recovery assay after viral internalization, suggesting that EHV-1 enters E. Derm cells via energy- and pH-dependent endocytosis. These results suggest that EHV-1 utilizes multiple endocytic pathways in different cell types to establish productive infection.
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spelling pubmed-71119962020-04-02 Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways Hasebe, Rie Sasaki, Michihito Sawa, Hirofumi Wada, Ryuichi Umemura, Takashi Kimura, Takashi Virology Article We investigated the mechanism by which equine herpesvirus-1 (EHV-1) enters primary cultured equine brain microvascular endothelial cells (EBMECs) and equine dermis (E. Derm) cells. EHV-1 colocalized with caveolin in EBMECs and the infection was greatly reduced by the expression of a dominant negative form of equine caveolin-1 (ecavY14F), suggesting that EHV-1 enters EBMECs via caveolar endocytosis. EHV-1 entry into E. Derm cells was significantly reduced by ATP depletion and treatments with lysosomotropic agents. Enveloped virions were detected from E. Derm cells by infectious virus recovery assay after viral internalization, suggesting that EHV-1 enters E. Derm cells via energy- and pH-dependent endocytosis. These results suggest that EHV-1 utilizes multiple endocytic pathways in different cell types to establish productive infection. Elsevier Inc. 2009-10-25 2009-08-31 /pmc/articles/PMC7111996/ /pubmed/19720389 http://dx.doi.org/10.1016/j.virol.2009.07.032 Text en Copyright © 2009 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hasebe, Rie
Sasaki, Michihito
Sawa, Hirofumi
Wada, Ryuichi
Umemura, Takashi
Kimura, Takashi
Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title_full Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title_fullStr Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title_full_unstemmed Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title_short Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
title_sort infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111996/
https://www.ncbi.nlm.nih.gov/pubmed/19720389
http://dx.doi.org/10.1016/j.virol.2009.07.032
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