Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus

Viral noncoding (nc) RNAs have been shown to play important roles in viral life cycle. Many viruses employ different mechanism to produce ncRNAs. Here, we report that coronavirus infectious bronchitis virus (IBV) produces a novel ncRNA in virus-infected cells. This ncRNA consists of 563 nucleotides...

Descripción completa

Detalles Bibliográficos
Autores principales: An, Hongliu, Cai, Zhichao, Yang, Yuying, Wang, Zhaoxiong, Liu, Ding Xiang, Fang, Shouguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Inc. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112027/
https://www.ncbi.nlm.nih.gov/pubmed/30616206
http://dx.doi.org/10.1016/j.virol.2018.12.019
_version_ 1783513402077872128
author An, Hongliu
Cai, Zhichao
Yang, Yuying
Wang, Zhaoxiong
Liu, Ding Xiang
Fang, Shouguo
author_facet An, Hongliu
Cai, Zhichao
Yang, Yuying
Wang, Zhaoxiong
Liu, Ding Xiang
Fang, Shouguo
author_sort An, Hongliu
collection PubMed
description Viral noncoding (nc) RNAs have been shown to play important roles in viral life cycle. Many viruses employ different mechanism to produce ncRNAs. Here, we report that coronavirus infectious bronchitis virus (IBV) produces a novel ncRNA in virus-infected cells. This ncRNA consists of 563 nucleotides excluding a poly(A) tail, is mainly derived from the 3′-untranslated region of IBV genome, and contains a 63-nt-long of terminal leader sequence derived from the 5′ end of the viral genome. Using mutagenesis and reverse genetics, we reveal that this ncRNA is a subgenomic RNA generated by discontinuous transcription mechanism.
format Online
Article
Text
id pubmed-7112027
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Authors. Published by Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-71120272020-04-02 Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus An, Hongliu Cai, Zhichao Yang, Yuying Wang, Zhaoxiong Liu, Ding Xiang Fang, Shouguo Virology Article Viral noncoding (nc) RNAs have been shown to play important roles in viral life cycle. Many viruses employ different mechanism to produce ncRNAs. Here, we report that coronavirus infectious bronchitis virus (IBV) produces a novel ncRNA in virus-infected cells. This ncRNA consists of 563 nucleotides excluding a poly(A) tail, is mainly derived from the 3′-untranslated region of IBV genome, and contains a 63-nt-long of terminal leader sequence derived from the 5′ end of the viral genome. Using mutagenesis and reverse genetics, we reveal that this ncRNA is a subgenomic RNA generated by discontinuous transcription mechanism. The Authors. Published by Elsevier Inc. 2019-02 2019-01-05 /pmc/articles/PMC7112027/ /pubmed/30616206 http://dx.doi.org/10.1016/j.virol.2018.12.019 Text en © 2018 The Authors. Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
An, Hongliu
Cai, Zhichao
Yang, Yuying
Wang, Zhaoxiong
Liu, Ding Xiang
Fang, Shouguo
Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title_full Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title_fullStr Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title_full_unstemmed Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title_short Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
title_sort identification and formation mechanism of a novel noncoding rna produced by avian infectious bronchitis virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112027/
https://www.ncbi.nlm.nih.gov/pubmed/30616206
http://dx.doi.org/10.1016/j.virol.2018.12.019
work_keys_str_mv AT anhongliu identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus
AT caizhichao identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus
AT yangyuying identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus
AT wangzhaoxiong identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus
AT liudingxiang identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus
AT fangshouguo identificationandformationmechanismofanovelnoncodingrnaproducedbyavianinfectiousbronchitisvirus

Ejemplares similares