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Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections

Coronaviruses belong to the family Coronaviridae, which primarily cause infection of the upper respiratory and gastrointestinal tract of hosts. Transmissible gastroenteritis virus (TGEV) is an economically significant coronavirus that can cause severe diarrhea in pigs. Silver nanomaterials (Ag NMs)...

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Detalles Bibliográficos
Autores principales: Lv, Xiaonan, Wang, Peng, Bai, Ru, Cong, Yingying, Suo, Siqingaowa, Ren, Xiaofeng, Chen, Chunying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112386/
https://www.ncbi.nlm.nih.gov/pubmed/24524838
http://dx.doi.org/10.1016/j.biomaterials.2014.01.054
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author Lv, Xiaonan
Wang, Peng
Bai, Ru
Cong, Yingying
Suo, Siqingaowa
Ren, Xiaofeng
Chen, Chunying
author_facet Lv, Xiaonan
Wang, Peng
Bai, Ru
Cong, Yingying
Suo, Siqingaowa
Ren, Xiaofeng
Chen, Chunying
author_sort Lv, Xiaonan
collection PubMed
description Coronaviruses belong to the family Coronaviridae, which primarily cause infection of the upper respiratory and gastrointestinal tract of hosts. Transmissible gastroenteritis virus (TGEV) is an economically significant coronavirus that can cause severe diarrhea in pigs. Silver nanomaterials (Ag NMs) have attracted great interests in recent years due to their excellent anti-microorganism properties. Herein, four representative Ag NMs including spherical Ag nanoparticles (Ag NPs, NM-300), two kinds of silver nanowires (XFJ011) and silver colloids (XFJ04) were selected to study their inhibitory effect on TGEV-induced host cell infection in vitro. Ag NPs were uniformly distributed, with particle sizes less than 20 nm by characterization of environmental scanning electron microscope and transmission electron microscope. Two types of silver nanowires were 60 nm and 400 nm in diameter, respectively. The average diameter of the silver colloids was approximately 10 nm. TGEV infection induced the occurring of apoptosis in swine testicle (ST) cells, down-regulated the expression of Bcl-2, up-regulated the expression of Bax, altered mitochondrial membrane potential, activated p38 MAPK signal pathway, and increased expression of p53 as evidenced by immunofluorescence assays, real-time PCR, flow cytometry and Western blot. Under non-toxic concentrations, Ag NPs and silver nanowires significantly diminished the infectivity of TGEV in ST cells. Moreover, further results showed that Ag NPs and silver nanowires decreased the number of apoptotic cells induced by TGEV through regulating p38/mitochondria-caspase-3 signaling pathway. Our data indicate that Ag NMs are effective in prevention of TGEV-mediated cell infection as a virucidal agent or as an inhibitor of viral entry and the present findings may provide new insights into antiviral therapy of coronaviruses.
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spelling pubmed-71123862020-04-02 Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections Lv, Xiaonan Wang, Peng Bai, Ru Cong, Yingying Suo, Siqingaowa Ren, Xiaofeng Chen, Chunying Biomaterials Article Coronaviruses belong to the family Coronaviridae, which primarily cause infection of the upper respiratory and gastrointestinal tract of hosts. Transmissible gastroenteritis virus (TGEV) is an economically significant coronavirus that can cause severe diarrhea in pigs. Silver nanomaterials (Ag NMs) have attracted great interests in recent years due to their excellent anti-microorganism properties. Herein, four representative Ag NMs including spherical Ag nanoparticles (Ag NPs, NM-300), two kinds of silver nanowires (XFJ011) and silver colloids (XFJ04) were selected to study their inhibitory effect on TGEV-induced host cell infection in vitro. Ag NPs were uniformly distributed, with particle sizes less than 20 nm by characterization of environmental scanning electron microscope and transmission electron microscope. Two types of silver nanowires were 60 nm and 400 nm in diameter, respectively. The average diameter of the silver colloids was approximately 10 nm. TGEV infection induced the occurring of apoptosis in swine testicle (ST) cells, down-regulated the expression of Bcl-2, up-regulated the expression of Bax, altered mitochondrial membrane potential, activated p38 MAPK signal pathway, and increased expression of p53 as evidenced by immunofluorescence assays, real-time PCR, flow cytometry and Western blot. Under non-toxic concentrations, Ag NPs and silver nanowires significantly diminished the infectivity of TGEV in ST cells. Moreover, further results showed that Ag NPs and silver nanowires decreased the number of apoptotic cells induced by TGEV through regulating p38/mitochondria-caspase-3 signaling pathway. Our data indicate that Ag NMs are effective in prevention of TGEV-mediated cell infection as a virucidal agent or as an inhibitor of viral entry and the present findings may provide new insights into antiviral therapy of coronaviruses. Elsevier Ltd. 2014-04 2014-02-10 /pmc/articles/PMC7112386/ /pubmed/24524838 http://dx.doi.org/10.1016/j.biomaterials.2014.01.054 Text en Copyright © 2014 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lv, Xiaonan
Wang, Peng
Bai, Ru
Cong, Yingying
Suo, Siqingaowa
Ren, Xiaofeng
Chen, Chunying
Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title_full Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title_fullStr Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title_full_unstemmed Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title_short Inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
title_sort inhibitory effect of silver nanomaterials on transmissible virus-induced host cell infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112386/
https://www.ncbi.nlm.nih.gov/pubmed/24524838
http://dx.doi.org/10.1016/j.biomaterials.2014.01.054
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