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Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis
The complex interplay between cytokines and chemokines regulates innate and adaptive immune responses against pathogens; specifically, cytokine and chemokine expression drives activation of immune effector cells and their recruitment to tissue infection sites. Herein, we inoculated dogs with Leishma...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112513/ https://www.ncbi.nlm.nih.gov/pubmed/23911411 http://dx.doi.org/10.1016/j.molimm.2013.05.231 |
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author | Vitoriano-Souza, Juliana Moreira, Nádia das Dores Menezes-Souza, Daniel Roatt, Bruno Mendes de Oliveira Aguiar-Soares, Rodrigo Dian Siqueira-Mathias, Fernando Augusto de Oliveira Cardoso, Jamille Mirelle Giunchetti, Rodolfo Cordeiro de Sá, Renata Guerra Corrêa-Oliveira, Rodrigo Carneiro, Cláudia Martins Reis, Alexandre Barbosa |
author_facet | Vitoriano-Souza, Juliana Moreira, Nádia das Dores Menezes-Souza, Daniel Roatt, Bruno Mendes de Oliveira Aguiar-Soares, Rodrigo Dian Siqueira-Mathias, Fernando Augusto de Oliveira Cardoso, Jamille Mirelle Giunchetti, Rodolfo Cordeiro de Sá, Renata Guerra Corrêa-Oliveira, Rodrigo Carneiro, Cláudia Martins Reis, Alexandre Barbosa |
author_sort | Vitoriano-Souza, Juliana |
collection | PubMed |
description | The complex interplay between cytokines and chemokines regulates innate and adaptive immune responses against pathogens; specifically, cytokine and chemokine expression drives activation of immune effector cells and their recruitment to tissue infection sites. Herein, we inoculated dogs with Leishmania braziliensis antigens plus saponin (the LBSap vaccine), as well as with the vaccine components, and then used real-time PCR to evaluate the kinetics of dermal expression of mRNAs of cytokines (IL-12, IFN-γ, TNF-α, IL-4, IL-13, TGF-β and IL-10) and chemokines (CCL2, CCL4, CCL5, CCL21 and CXCL8) 1, 12, 24 and 48 h after inoculation. We also evaluated the correlation between cytokine and chemokine expression and dermal cellularity. The LBSap vaccine induced high levels of IL-12 and IL-10 expression at 12 and 24 h, respectively. Furthermore, we observed positive correlations between IL-12 and IL-13 expression, IFN-γ and IL-13 expression, and IL-13 and TGF-β expression, suggesting that a mixed cytokine microenvironment developed after immunization with the vaccine. Inoculation with the saponin adjuvant alone induced a chemokine and cytokine expression profile similar to that observed in the LBSap group. CCL4 and CXCL8 chemokine expression was up regulated by the LBSap vaccine. CCL5 expression was initially highest in the LBSap group, but at 48 h, expression was highest in the LB group. Information about the kinetics of the immune response to this vaccine gained using this dog model will help to elucidate the mechanisms of and factors involved in a protective response against Leishmania infection and will aid in establishing rational approaches for the development of vaccines against canine visceral leishmaniasis. |
format | Online Article Text |
id | pubmed-7112513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71125132020-04-02 Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis Vitoriano-Souza, Juliana Moreira, Nádia das Dores Menezes-Souza, Daniel Roatt, Bruno Mendes de Oliveira Aguiar-Soares, Rodrigo Dian Siqueira-Mathias, Fernando Augusto de Oliveira Cardoso, Jamille Mirelle Giunchetti, Rodolfo Cordeiro de Sá, Renata Guerra Corrêa-Oliveira, Rodrigo Carneiro, Cláudia Martins Reis, Alexandre Barbosa Mol Immunol Article The complex interplay between cytokines and chemokines regulates innate and adaptive immune responses against pathogens; specifically, cytokine and chemokine expression drives activation of immune effector cells and their recruitment to tissue infection sites. Herein, we inoculated dogs with Leishmania braziliensis antigens plus saponin (the LBSap vaccine), as well as with the vaccine components, and then used real-time PCR to evaluate the kinetics of dermal expression of mRNAs of cytokines (IL-12, IFN-γ, TNF-α, IL-4, IL-13, TGF-β and IL-10) and chemokines (CCL2, CCL4, CCL5, CCL21 and CXCL8) 1, 12, 24 and 48 h after inoculation. We also evaluated the correlation between cytokine and chemokine expression and dermal cellularity. The LBSap vaccine induced high levels of IL-12 and IL-10 expression at 12 and 24 h, respectively. Furthermore, we observed positive correlations between IL-12 and IL-13 expression, IFN-γ and IL-13 expression, and IL-13 and TGF-β expression, suggesting that a mixed cytokine microenvironment developed after immunization with the vaccine. Inoculation with the saponin adjuvant alone induced a chemokine and cytokine expression profile similar to that observed in the LBSap group. CCL4 and CXCL8 chemokine expression was up regulated by the LBSap vaccine. CCL5 expression was initially highest in the LBSap group, but at 48 h, expression was highest in the LB group. Information about the kinetics of the immune response to this vaccine gained using this dog model will help to elucidate the mechanisms of and factors involved in a protective response against Leishmania infection and will aid in establishing rational approaches for the development of vaccines against canine visceral leishmaniasis. Elsevier Ltd. 2013-12-31 2013-08-01 /pmc/articles/PMC7112513/ /pubmed/23911411 http://dx.doi.org/10.1016/j.molimm.2013.05.231 Text en Copyright © 2013 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Vitoriano-Souza, Juliana Moreira, Nádia das Dores Menezes-Souza, Daniel Roatt, Bruno Mendes de Oliveira Aguiar-Soares, Rodrigo Dian Siqueira-Mathias, Fernando Augusto de Oliveira Cardoso, Jamille Mirelle Giunchetti, Rodolfo Cordeiro de Sá, Renata Guerra Corrêa-Oliveira, Rodrigo Carneiro, Cláudia Martins Reis, Alexandre Barbosa Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title | Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title_full | Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title_fullStr | Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title_full_unstemmed | Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title_short | Dogs immunized with LBSap vaccine displayed high levels of IL-12 and IL-10 cytokines and CCL4, CCL5 and CXCL8 chemokines in the dermis |
title_sort | dogs immunized with lbsap vaccine displayed high levels of il-12 and il-10 cytokines and ccl4, ccl5 and cxcl8 chemokines in the dermis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112513/ https://www.ncbi.nlm.nih.gov/pubmed/23911411 http://dx.doi.org/10.1016/j.molimm.2013.05.231 |
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