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Induction and sequencing of Rousette bat interferon α and β genes
Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112530/ https://www.ncbi.nlm.nih.gov/pubmed/18436311 http://dx.doi.org/10.1016/j.vetimm.2008.03.004 |
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author | Omatsu, Tsutomu Bak, Eun-Jung Ishii, Yoshiyuki Kyuwa, Shigeru Tohya, Yukinobu Akashi, Hiroomi Yoshikawa, Yasuhiro |
author_facet | Omatsu, Tsutomu Bak, Eun-Jung Ishii, Yoshiyuki Kyuwa, Shigeru Tohya, Yukinobu Akashi, Hiroomi Yoshikawa, Yasuhiro |
author_sort | Omatsu, Tsutomu |
collection | PubMed |
description | Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon viral infection in bats, the nucleic acid, and amino acid sequences of Egyptian Rousette (Rousettus aegyptiacus) IFN-α and -β were characterized. Sequence data indicated that bat IFN-α consists of 562-bp encoded 187-aa, and IFN-β consisted of 558-bp encoded 186-aa. Phylogenetic analysis of the overall identity of IFN-β shared the highest sequence homology with pig IFN-β in both nucleotide and amino acid level. Stimulation of bat primary kidney cells (BPKCs) and bat lung cell lines, Tb-1 Lu, with polyinosinic–polycytidylic acid (poly(I:C)) or exogenous bat type I IFNs resulted in increased type I IFNs mRNA expression in BPKCs, but not in Tb-1 Lu. Characterization of the bat IFN-α and -β genes allows understanding of the immune responses upon stimulation in different tissues, thus providing practical strategies for control and treatment of clinically important diseases. These results are important especially for the virus infection, and suggest that future molecular studies on virus infection experiment of bats in vitro will require careful consideration of the differences of type I IFN expression patterns in different cell types. |
format | Online Article Text |
id | pubmed-7112530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71125302020-04-02 Induction and sequencing of Rousette bat interferon α and β genes Omatsu, Tsutomu Bak, Eun-Jung Ishii, Yoshiyuki Kyuwa, Shigeru Tohya, Yukinobu Akashi, Hiroomi Yoshikawa, Yasuhiro Vet Immunol Immunopathol Article Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon viral infection in bats, the nucleic acid, and amino acid sequences of Egyptian Rousette (Rousettus aegyptiacus) IFN-α and -β were characterized. Sequence data indicated that bat IFN-α consists of 562-bp encoded 187-aa, and IFN-β consisted of 558-bp encoded 186-aa. Phylogenetic analysis of the overall identity of IFN-β shared the highest sequence homology with pig IFN-β in both nucleotide and amino acid level. Stimulation of bat primary kidney cells (BPKCs) and bat lung cell lines, Tb-1 Lu, with polyinosinic–polycytidylic acid (poly(I:C)) or exogenous bat type I IFNs resulted in increased type I IFNs mRNA expression in BPKCs, but not in Tb-1 Lu. Characterization of the bat IFN-α and -β genes allows understanding of the immune responses upon stimulation in different tissues, thus providing practical strategies for control and treatment of clinically important diseases. These results are important especially for the virus infection, and suggest that future molecular studies on virus infection experiment of bats in vitro will require careful consideration of the differences of type I IFN expression patterns in different cell types. Elsevier B.V. 2008-07-15 2008-03-21 /pmc/articles/PMC7112530/ /pubmed/18436311 http://dx.doi.org/10.1016/j.vetimm.2008.03.004 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Omatsu, Tsutomu Bak, Eun-Jung Ishii, Yoshiyuki Kyuwa, Shigeru Tohya, Yukinobu Akashi, Hiroomi Yoshikawa, Yasuhiro Induction and sequencing of Rousette bat interferon α and β genes |
title | Induction and sequencing of Rousette bat interferon α and β genes |
title_full | Induction and sequencing of Rousette bat interferon α and β genes |
title_fullStr | Induction and sequencing of Rousette bat interferon α and β genes |
title_full_unstemmed | Induction and sequencing of Rousette bat interferon α and β genes |
title_short | Induction and sequencing of Rousette bat interferon α and β genes |
title_sort | induction and sequencing of rousette bat interferon α and β genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112530/ https://www.ncbi.nlm.nih.gov/pubmed/18436311 http://dx.doi.org/10.1016/j.vetimm.2008.03.004 |
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