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Induction and sequencing of Rousette bat interferon α and β genes

Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon...

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Autores principales: Omatsu, Tsutomu, Bak, Eun-Jung, Ishii, Yoshiyuki, Kyuwa, Shigeru, Tohya, Yukinobu, Akashi, Hiroomi, Yoshikawa, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112530/
https://www.ncbi.nlm.nih.gov/pubmed/18436311
http://dx.doi.org/10.1016/j.vetimm.2008.03.004
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author Omatsu, Tsutomu
Bak, Eun-Jung
Ishii, Yoshiyuki
Kyuwa, Shigeru
Tohya, Yukinobu
Akashi, Hiroomi
Yoshikawa, Yasuhiro
author_facet Omatsu, Tsutomu
Bak, Eun-Jung
Ishii, Yoshiyuki
Kyuwa, Shigeru
Tohya, Yukinobu
Akashi, Hiroomi
Yoshikawa, Yasuhiro
author_sort Omatsu, Tsutomu
collection PubMed
description Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon viral infection in bats, the nucleic acid, and amino acid sequences of Egyptian Rousette (Rousettus aegyptiacus) IFN-α and -β were characterized. Sequence data indicated that bat IFN-α consists of 562-bp encoded 187-aa, and IFN-β consisted of 558-bp encoded 186-aa. Phylogenetic analysis of the overall identity of IFN-β shared the highest sequence homology with pig IFN-β in both nucleotide and amino acid level. Stimulation of bat primary kidney cells (BPKCs) and bat lung cell lines, Tb-1 Lu, with polyinosinic–polycytidylic acid (poly(I:C)) or exogenous bat type I IFNs resulted in increased type I IFNs mRNA expression in BPKCs, but not in Tb-1 Lu. Characterization of the bat IFN-α and -β genes allows understanding of the immune responses upon stimulation in different tissues, thus providing practical strategies for control and treatment of clinically important diseases. These results are important especially for the virus infection, and suggest that future molecular studies on virus infection experiment of bats in vitro will require careful consideration of the differences of type I IFN expression patterns in different cell types.
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spelling pubmed-71125302020-04-02 Induction and sequencing of Rousette bat interferon α and β genes Omatsu, Tsutomu Bak, Eun-Jung Ishii, Yoshiyuki Kyuwa, Shigeru Tohya, Yukinobu Akashi, Hiroomi Yoshikawa, Yasuhiro Vet Immunol Immunopathol Article Bats are considered to be natural reservoirs for several viruses of clinical importance, including rabies virus, Nipah virus, and Hendra virus. Type I interferons (IFNs) is an important part of the immune system in the defense against viral infection. To investigate the function of type I IFNs upon viral infection in bats, the nucleic acid, and amino acid sequences of Egyptian Rousette (Rousettus aegyptiacus) IFN-α and -β were characterized. Sequence data indicated that bat IFN-α consists of 562-bp encoded 187-aa, and IFN-β consisted of 558-bp encoded 186-aa. Phylogenetic analysis of the overall identity of IFN-β shared the highest sequence homology with pig IFN-β in both nucleotide and amino acid level. Stimulation of bat primary kidney cells (BPKCs) and bat lung cell lines, Tb-1 Lu, with polyinosinic–polycytidylic acid (poly(I:C)) or exogenous bat type I IFNs resulted in increased type I IFNs mRNA expression in BPKCs, but not in Tb-1 Lu. Characterization of the bat IFN-α and -β genes allows understanding of the immune responses upon stimulation in different tissues, thus providing practical strategies for control and treatment of clinically important diseases. These results are important especially for the virus infection, and suggest that future molecular studies on virus infection experiment of bats in vitro will require careful consideration of the differences of type I IFN expression patterns in different cell types. Elsevier B.V. 2008-07-15 2008-03-21 /pmc/articles/PMC7112530/ /pubmed/18436311 http://dx.doi.org/10.1016/j.vetimm.2008.03.004 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Omatsu, Tsutomu
Bak, Eun-Jung
Ishii, Yoshiyuki
Kyuwa, Shigeru
Tohya, Yukinobu
Akashi, Hiroomi
Yoshikawa, Yasuhiro
Induction and sequencing of Rousette bat interferon α and β genes
title Induction and sequencing of Rousette bat interferon α and β genes
title_full Induction and sequencing of Rousette bat interferon α and β genes
title_fullStr Induction and sequencing of Rousette bat interferon α and β genes
title_full_unstemmed Induction and sequencing of Rousette bat interferon α and β genes
title_short Induction and sequencing of Rousette bat interferon α and β genes
title_sort induction and sequencing of rousette bat interferon α and β genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112530/
https://www.ncbi.nlm.nih.gov/pubmed/18436311
http://dx.doi.org/10.1016/j.vetimm.2008.03.004
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