Identification of cell lines permissive for human coronavirus NL63

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was obser...

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Autores principales: Schildgen, Oliver, Jebbink, Maarten F., de Vries, Michel, Pyrc, Krzysztov, Dijkman, Ronald, Simon, Arne, Müller, Andreas, Kupfer, Bernd, van der Hoek, Lia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2006
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112796/
https://www.ncbi.nlm.nih.gov/pubmed/16962670
http://dx.doi.org/10.1016/j.jviromet.2006.07.023
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author Schildgen, Oliver
Jebbink, Maarten F.
de Vries, Michel
Pyrc, Krzysztov
Dijkman, Ronald
Simon, Arne
Müller, Andreas
Kupfer, Bernd
van der Hoek, Lia
author_facet Schildgen, Oliver
Jebbink, Maarten F.
de Vries, Michel
Pyrc, Krzysztov
Dijkman, Ronald
Simon, Arne
Müller, Andreas
Kupfer, Bernd
van der Hoek, Lia
author_sort Schildgen, Oliver
collection PubMed
description Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin–Darby–Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63.
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spelling pubmed-71127962020-04-02 Identification of cell lines permissive for human coronavirus NL63 Schildgen, Oliver Jebbink, Maarten F. de Vries, Michel Pyrc, Krzysztov Dijkman, Ronald Simon, Arne Müller, Andreas Kupfer, Bernd van der Hoek, Lia J Virol Methods Short Communication Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin–Darby–Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63. Elsevier B.V. 2006-12 2006-09-07 /pmc/articles/PMC7112796/ /pubmed/16962670 http://dx.doi.org/10.1016/j.jviromet.2006.07.023 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Schildgen, Oliver
Jebbink, Maarten F.
de Vries, Michel
Pyrc, Krzysztov
Dijkman, Ronald
Simon, Arne
Müller, Andreas
Kupfer, Bernd
van der Hoek, Lia
Identification of cell lines permissive for human coronavirus NL63
title Identification of cell lines permissive for human coronavirus NL63
title_full Identification of cell lines permissive for human coronavirus NL63
title_fullStr Identification of cell lines permissive for human coronavirus NL63
title_full_unstemmed Identification of cell lines permissive for human coronavirus NL63
title_short Identification of cell lines permissive for human coronavirus NL63
title_sort identification of cell lines permissive for human coronavirus nl63
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112796/
https://www.ncbi.nlm.nih.gov/pubmed/16962670
http://dx.doi.org/10.1016/j.jviromet.2006.07.023
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