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Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()

Human coronaviruses are one of the main causes of upper respiratory tract infections in humans. While more often responsible for mild illness, they have been associated with illnesses that require hospitalization. In this study, an assay for one of the human coronaviruses, OC43, was developed using...

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Detalles Bibliográficos
Autores principales: Blanchard, Elisabeth G., Miao, Congrong, Haupt, Thomas E., Anderson, Larry J., Haynes, Lia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112803/
https://www.ncbi.nlm.nih.gov/pubmed/21801752
http://dx.doi.org/10.1016/j.jviromet.2011.07.005
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author Blanchard, Elisabeth G.
Miao, Congrong
Haupt, Thomas E.
Anderson, Larry J.
Haynes, Lia M.
author_facet Blanchard, Elisabeth G.
Miao, Congrong
Haupt, Thomas E.
Anderson, Larry J.
Haynes, Lia M.
author_sort Blanchard, Elisabeth G.
collection PubMed
description Human coronaviruses are one of the main causes of upper respiratory tract infections in humans. While more often responsible for mild illness, they have been associated with illnesses that require hospitalization. In this study, an assay for one of the human coronaviruses, OC43, was developed using a truncated recombinant nucleocapsid (N) protein antigen in an enzyme immunosorbent assay (ELISA) and evaluated using serum collected from HCoV-OC43-infected patients, healthy adults, and patients with other respiratory virus infections. Results showed that the diagnostic sensitivity and specificity of the assay were 90.9% (10/11) and 82.9% (39/47), respectively. To evaluate the clinical utility of the ELISA, serum samples collected from patients during an outbreak of HCoV-OC43 infection and previously identified as positive by HCoV-OC43 whole N ELISA were screened resulting in 100% diagnosis agreement between the testing methods. These results suggest that this assay offers a reliable method to detect HCoV-OC43 infection and may be a useful tool in coronavirus seroepidemiological studies.
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spelling pubmed-71128032020-04-02 Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43() Blanchard, Elisabeth G. Miao, Congrong Haupt, Thomas E. Anderson, Larry J. Haynes, Lia M. J Virol Methods Article Human coronaviruses are one of the main causes of upper respiratory tract infections in humans. While more often responsible for mild illness, they have been associated with illnesses that require hospitalization. In this study, an assay for one of the human coronaviruses, OC43, was developed using a truncated recombinant nucleocapsid (N) protein antigen in an enzyme immunosorbent assay (ELISA) and evaluated using serum collected from HCoV-OC43-infected patients, healthy adults, and patients with other respiratory virus infections. Results showed that the diagnostic sensitivity and specificity of the assay were 90.9% (10/11) and 82.9% (39/47), respectively. To evaluate the clinical utility of the ELISA, serum samples collected from patients during an outbreak of HCoV-OC43 infection and previously identified as positive by HCoV-OC43 whole N ELISA were screened resulting in 100% diagnosis agreement between the testing methods. These results suggest that this assay offers a reliable method to detect HCoV-OC43 infection and may be a useful tool in coronavirus seroepidemiological studies. Elsevier/North-Holland Biomedical Press 2011-10 2011-07-21 /pmc/articles/PMC7112803/ /pubmed/21801752 http://dx.doi.org/10.1016/j.jviromet.2011.07.005 Text en Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Blanchard, Elisabeth G.
Miao, Congrong
Haupt, Thomas E.
Anderson, Larry J.
Haynes, Lia M.
Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title_full Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title_fullStr Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title_full_unstemmed Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title_short Development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus OC43()
title_sort development of a recombinant truncated nucleocapsid protein based immunoassay for detection of antibodies against human coronavirus oc43()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112803/
https://www.ncbi.nlm.nih.gov/pubmed/21801752
http://dx.doi.org/10.1016/j.jviromet.2011.07.005
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