Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay

The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as we...

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Autores principales: Adachi, D., Johnson, G., Draker, R., Ayers, M., Mazzulli, T., Talbot, P.J., Tellier, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2004
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112817/
https://www.ncbi.nlm.nih.gov/pubmed/15488617
http://dx.doi.org/10.1016/j.jviromet.2004.07.008
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author Adachi, D.
Johnson, G.
Draker, R.
Ayers, M.
Mazzulli, T.
Talbot, P.J.
Tellier, R.
author_facet Adachi, D.
Johnson, G.
Draker, R.
Ayers, M.
Mazzulli, T.
Talbot, P.J.
Tellier, R.
author_sort Adachi, D.
collection PubMed
description The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.
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spelling pubmed-71128172020-04-02 Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay Adachi, D. Johnson, G. Draker, R. Ayers, M. Mazzulli, T. Talbot, P.J. Tellier, R. J Virol Methods Article The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada. Elsevier B.V. 2004-12-01 2004-09-11 /pmc/articles/PMC7112817/ /pubmed/15488617 http://dx.doi.org/10.1016/j.jviromet.2004.07.008 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Adachi, D.
Johnson, G.
Draker, R.
Ayers, M.
Mazzulli, T.
Talbot, P.J.
Tellier, R.
Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title_full Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title_fullStr Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title_full_unstemmed Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title_short Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay
title_sort comprehensive detection and identification of human coronaviruses, including the sars-associated coronavirus, with a single rt-pcr assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112817/
https://www.ncbi.nlm.nih.gov/pubmed/15488617
http://dx.doi.org/10.1016/j.jviromet.2004.07.008
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