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Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay

A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of bovine coronavirus (BCoV) RNA in clinical samples is described. The assay is based on TaqMan technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds sel...

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Detalles Bibliográficos
Autores principales:	Decaro, Nicola, Elia, Gabriella, Campolo, Marco, Desario, Costantina, Mari, Viviana, Radogna, Arianna, Colaianni, Maria Loredana, Cirone, Francesco, Tempesta, Maria, Buonavoglia, Canio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2008
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112840/ https://www.ncbi.nlm.nih.gov/pubmed/18579223 http://dx.doi.org/10.1016/j.jviromet.2008.05.016

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