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Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus
Canine respiratory coronavirus (CRCoV) has been detected recently in dogs with canine infectious respiratory disease and is involved in the clinical disease complex. CRCoV is a group 2 coronavirus most closely related to bovine coronavirus and human coronavirus OC43. A real-time PCR assay was develo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112842/ https://www.ncbi.nlm.nih.gov/pubmed/19013196 http://dx.doi.org/10.1016/j.jviromet.2008.10.008 |
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author | Mitchell, Judy A. Brooks, Harriet Shiu, Kai-Biu Brownlie, Joe Erles, Kerstin |
author_facet | Mitchell, Judy A. Brooks, Harriet Shiu, Kai-Biu Brownlie, Joe Erles, Kerstin |
author_sort | Mitchell, Judy A. |
collection | PubMed |
description | Canine respiratory coronavirus (CRCoV) has been detected recently in dogs with canine infectious respiratory disease and is involved in the clinical disease complex. CRCoV is a group 2 coronavirus most closely related to bovine coronavirus and human coronavirus OC43. A real-time PCR assay was developed for the detection of CRCoV. The assay was validated against cell culture grown virus and shown to have a high level of sensitivity. A range of tissue samples were collected from dogs at a re-homing centre with a history of endemic respiratory disease. The samples were tested using a conventional nested PCR assay and CRCoV was quantitated by real-time PCR. CRCoV was detected most frequently in the nasal mucosa, nasal tonsil and trachea. It was also detected in the lung, and bronchial lymph node. Of the enteric tissues, only one mesenteric lymph node sample was positive. In addition two colon samples were positive for CRCoV by nested PCR only. In conclusion, CRCoV appears to infect the upper respiratory tract preferentially. The CRCoV real-time PCR assay has proved to be a highly specific and sensitive assay that can be applied for diagnostic purposes as well as to investigate further the tissue tropism of CRCoV. |
format | Online Article Text |
id | pubmed-7112842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71128422020-04-02 Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus Mitchell, Judy A. Brooks, Harriet Shiu, Kai-Biu Brownlie, Joe Erles, Kerstin J Virol Methods Article Canine respiratory coronavirus (CRCoV) has been detected recently in dogs with canine infectious respiratory disease and is involved in the clinical disease complex. CRCoV is a group 2 coronavirus most closely related to bovine coronavirus and human coronavirus OC43. A real-time PCR assay was developed for the detection of CRCoV. The assay was validated against cell culture grown virus and shown to have a high level of sensitivity. A range of tissue samples were collected from dogs at a re-homing centre with a history of endemic respiratory disease. The samples were tested using a conventional nested PCR assay and CRCoV was quantitated by real-time PCR. CRCoV was detected most frequently in the nasal mucosa, nasal tonsil and trachea. It was also detected in the lung, and bronchial lymph node. Of the enteric tissues, only one mesenteric lymph node sample was positive. In addition two colon samples were positive for CRCoV by nested PCR only. In conclusion, CRCoV appears to infect the upper respiratory tract preferentially. The CRCoV real-time PCR assay has proved to be a highly specific and sensitive assay that can be applied for diagnostic purposes as well as to investigate further the tissue tropism of CRCoV. Elsevier B.V. 2009-02 2008-11-28 /pmc/articles/PMC7112842/ /pubmed/19013196 http://dx.doi.org/10.1016/j.jviromet.2008.10.008 Text en Copyright © 2008 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mitchell, Judy A. Brooks, Harriet Shiu, Kai-Biu Brownlie, Joe Erles, Kerstin Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title | Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title_full | Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title_fullStr | Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title_full_unstemmed | Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title_short | Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus |
title_sort | development of a quantitative real-time pcr for the detection of canine respiratory coronavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112842/ https://www.ncbi.nlm.nih.gov/pubmed/19013196 http://dx.doi.org/10.1016/j.jviromet.2008.10.008 |
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