A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens

A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3) PFU...

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Autores principales: Zhang, M., Yang, J.X., Lin, X.M., Zhu, C.H., He, J.Q., Liu, H., Lin, T.L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112847/
https://www.ncbi.nlm.nih.gov/pubmed/20399233
http://dx.doi.org/10.1016/j.jviromet.2010.04.004
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author Zhang, M.
Yang, J.X.
Lin, X.M.
Zhu, C.H.
He, J.Q.
Liu, H.
Lin, T.L.
author_facet Zhang, M.
Yang, J.X.
Lin, X.M.
Zhu, C.H.
He, J.Q.
Liu, H.
Lin, T.L.
author_sort Zhang, M.
collection PubMed
description A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3) PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection of STIV. In clinical tests, 128 samples isolated from pond-reared turtles were subjected to DAS-ELISA and PCR. The overall agreement between the results obtained by DAS-ELISA and PCR was 98.4%. The results indicate that the DAS-ELISA method could be used for diagnosing diseases caused by STIV.
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spelling pubmed-71128472020-04-02 A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens Zhang, M. Yang, J.X. Lin, X.M. Zhu, C.H. He, J.Q. Liu, H. Lin, T.L. J Virol Methods Article A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of the soft-shelled turtle iridovirus (STIV) was developed using a specific monoclonal antibody (mAb) against STIV and anti-STIV rabbit serum. Using DAS-ELISA, the detection limit of STIV was found to be 10(3) PFU/ml. The positive rate of 15 STIV samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. These data show that DAS-ELISA is highly specific and sensitive for the detection of STIV. In clinical tests, 128 samples isolated from pond-reared turtles were subjected to DAS-ELISA and PCR. The overall agreement between the results obtained by DAS-ELISA and PCR was 98.4%. The results indicate that the DAS-ELISA method could be used for diagnosing diseases caused by STIV. Elsevier B.V. 2010-08 2010-04-16 /pmc/articles/PMC7112847/ /pubmed/20399233 http://dx.doi.org/10.1016/j.jviromet.2010.04.004 Text en Copyright © 2010 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhang, M.
Yang, J.X.
Lin, X.M.
Zhu, C.H.
He, J.Q.
Liu, H.
Lin, T.L.
A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title_full A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title_fullStr A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title_full_unstemmed A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title_short A double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
title_sort double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112847/
https://www.ncbi.nlm.nih.gov/pubmed/20399233
http://dx.doi.org/10.1016/j.jviromet.2010.04.004
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