Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR

A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was u...

Descripción completa

Detalles Bibliográficos
Autores principales: Elia, Gabriella, Cavalli, Alessandra, Desario, Costantina, Lorusso, Eleonora, Lucente, Maria Stella, Decaro, Nicola, Martella, Vito, Buonavoglia, Canio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112852/
https://www.ncbi.nlm.nih.gov/pubmed/17692932
http://dx.doi.org/10.1016/j.jviromet.2007.06.017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112852/
https://www.ncbi.nlm.nih.gov/pubmed/17692932
http://dx.doi.org/10.1016/j.jviromet.2007.06.017

Ejemplares similares