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Development of duplex real-time PCR for detection of two DNA respiratory viruses
A method was developed for the detection and quantitation of HAdV (human adenovirus) and HBoV (human bocavirus) based on a duplex real-time PCR, the AB PCR, using a Smartcycler instrument. A control real-time PCR was carried out on albumin DNA to standardise the non-homogenous respiratory samples. N...
Autores principales: | Dina, Julia, Nguyen, Emilie, Gouarin, Stephanie, Petitjean, Joelle, Parienti, Jean-Jacques, Nimal, Delphine, Brouard, Jacques, Freymuth, François, Vabret, Astrid |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112853/ https://www.ncbi.nlm.nih.gov/pubmed/19654024 http://dx.doi.org/10.1016/j.jviromet.2009.07.025 |
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