A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67 × 10(0) copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detect...

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Autores principales: Fan, Qing, Xie, Zhixun, Xie, Liji, Liu, Jiabo, Pang, Yaoshan, Deng, Xianwen, Xie, Zhiqin, Peng, Yi, Wang, Xiuqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. Published by Elsevier B.V. 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112856/
https://www.ncbi.nlm.nih.gov/pubmed/22947692
http://dx.doi.org/10.1016/j.jviromet.2012.08.007
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author Fan, Qing
Xie, Zhixun
Xie, Liji
Liu, Jiabo
Pang, Yaoshan
Deng, Xianwen
Xie, Zhiqin
Peng, Yi
Wang, Xiuqing
author_facet Fan, Qing
Xie, Zhixun
Xie, Liji
Liu, Jiabo
Pang, Yaoshan
Deng, Xianwen
Xie, Zhiqin
Peng, Yi
Wang, Xiuqing
author_sort Fan, Qing
collection PubMed
description A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67 × 10(0) copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
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spelling pubmed-71128562020-04-02 A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus Fan, Qing Xie, Zhixun Xie, Liji Liu, Jiabo Pang, Yaoshan Deng, Xianwen Xie, Zhiqin Peng, Yi Wang, Xiuqing J Virol Methods Article A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67 × 10(0) copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples. Elsevier B.V. Published by Elsevier B.V. 2012-12 2012-08-27 /pmc/articles/PMC7112856/ /pubmed/22947692 http://dx.doi.org/10.1016/j.jviromet.2012.08.007 Text en Copyright © 2012 Elsevier B.V. Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fan, Qing
Xie, Zhixun
Xie, Liji
Liu, Jiabo
Pang, Yaoshan
Deng, Xianwen
Xie, Zhiqin
Peng, Yi
Wang, Xiuqing
A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title_full A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title_fullStr A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title_full_unstemmed A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title_short A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
title_sort reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112856/
https://www.ncbi.nlm.nih.gov/pubmed/22947692
http://dx.doi.org/10.1016/j.jviromet.2012.08.007
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