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Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43

The nucleocapsid (N) gene of human coronavirus strain OC43 (HCoV-OC43) was amplified by reverse transcriptase-polymerase chain reaction, and cloned in pENTR™/D-TOPO(®) plasmid. This plasmid containing the N gene was recombined with in a BaculoDirect™ baculovirus DNA designed in order to express N pr...

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Detalles Bibliográficos
Autores principales:	Mourez, Thomas, Vabret, Astrid, Han, Yang, Dina, Julia, Legrand, Loïc, Corbet, Sandrine, Freymuth, François
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2007
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112889/ https://www.ncbi.nlm.nih.gov/pubmed/17079026 http://dx.doi.org/10.1016/j.jviromet.2006.09.024

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