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Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection
A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112893/ https://www.ncbi.nlm.nih.gov/pubmed/15620403 http://dx.doi.org/10.1016/j.jviromet.2004.09.017 |
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author | Britton, Paul Evans, Sharon Dove, Brian Davies, Marc Casais, Rosa Cavanagh, Dave |
author_facet | Britton, Paul Evans, Sharon Dove, Brian Davies, Marc Casais, Rosa Cavanagh, Dave |
author_sort | Britton, Paul |
collection | PubMed |
description | A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel, V., Siddell, S.G., Cavanagh, D., Britton, P., 2001. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75, 12359–12369]. The method has subsequently been used to generate a recombinant IBV expressing a chimaeric S gene [Casais, R., Dove, B., Cavanagh, D., Britton, P., 2003. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol. 77, 9084–9089]. Use of vaccinia virus as a vector for the full-length cDNA of the IBV genome has the advantage that modifications can be made to the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. We describe the use of homologous recombination as a method for modifying the Beaudette full-length cDNA, within the vaccinia virus genome, without the requirement for in vitro assembly of the IBV cDNA. To demonstrate the feasibility of the method we exchanged the ectodomain of the Beaudette spike gene for the corresponding region from IBV M41 and generated two recombinant infectious bronchitis viruses (rIBVs) expressing the chimaeric S protein, validating the method as an alternative way for generating rIBVs. |
format | Online Article Text |
id | pubmed-7112893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71128932020-04-02 Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection Britton, Paul Evans, Sharon Dove, Brian Davies, Marc Casais, Rosa Cavanagh, Dave J Virol Methods Article A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel, V., Siddell, S.G., Cavanagh, D., Britton, P., 2001. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75, 12359–12369]. The method has subsequently been used to generate a recombinant IBV expressing a chimaeric S gene [Casais, R., Dove, B., Cavanagh, D., Britton, P., 2003. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol. 77, 9084–9089]. Use of vaccinia virus as a vector for the full-length cDNA of the IBV genome has the advantage that modifications can be made to the IBV cDNA using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. We describe the use of homologous recombination as a method for modifying the Beaudette full-length cDNA, within the vaccinia virus genome, without the requirement for in vitro assembly of the IBV cDNA. To demonstrate the feasibility of the method we exchanged the ectodomain of the Beaudette spike gene for the corresponding region from IBV M41 and generated two recombinant infectious bronchitis viruses (rIBVs) expressing the chimaeric S protein, validating the method as an alternative way for generating rIBVs. Elsevier B.V. 2005-02 2004-11-05 /pmc/articles/PMC7112893/ /pubmed/15620403 http://dx.doi.org/10.1016/j.jviromet.2004.09.017 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Britton, Paul Evans, Sharon Dove, Brian Davies, Marc Casais, Rosa Cavanagh, Dave Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title | Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title_full | Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title_fullStr | Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title_full_unstemmed | Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title_short | Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
title_sort | generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112893/ https://www.ncbi.nlm.nih.gov/pubmed/15620403 http://dx.doi.org/10.1016/j.jviromet.2004.09.017 |
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