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Development of a one-step real-time quantitative PCR assay based on primer-probe energy transfer for the detection of porcine reproductive and respiratory syndrome virus

A one-step real-time RT-PCR method has been developed for the simultaneous detection of both genotypes of porcine reproductive and respiratory syndrome virus (PRRSV). The assay is based on primer-probe energy transfer, and the most important advantage of this is the relative tolerance towards mutati...

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Detalles Bibliográficos
Autores principales:	Balka, Gyula, Hornyák, Ákos, Bálint, Ádám, Benyeda, Zsófia, Rusvai, Miklós
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2009
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112897/ https://www.ncbi.nlm.nih.gov/pubmed/19200443 http://dx.doi.org/10.1016/j.jviromet.2009.01.014

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