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A long distance RT-PCR able to amplify the Pestivirus genome
A method to amplify long genomic regions (up to ∼12.3 kb) from pestiviruses in one RT-PCR is described. The difficulty in designing conserved Pestivirus primers for the amplification of genomes from highly divergent isolates simply by means of overlapping segments is demonstrated using new bioinform...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112918/ https://www.ncbi.nlm.nih.gov/pubmed/16497393 http://dx.doi.org/10.1016/j.jviromet.2006.01.005 |