Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method...

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Detalles Bibliográficos
Autores principales: Chi, X. Sherry, Li, Fenglan, Tam, John S., Rappaport, Ruth, Cheng, Sheau-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112931/
https://www.ncbi.nlm.nih.gov/pubmed/17030067
http://dx.doi.org/10.1016/j.jviromet.2006.08.015
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author Chi, X. Sherry
Li, Fenglan
Tam, John S.
Rappaport, Ruth
Cheng, Sheau-Mei
author_facet Chi, X. Sherry
Li, Fenglan
Tam, John S.
Rappaport, Ruth
Cheng, Sheau-Mei
author_sort Chi, X. Sherry
collection PubMed
description A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens.
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spelling pubmed-71129312020-04-02 Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses Chi, X. Sherry Li, Fenglan Tam, John S. Rappaport, Ruth Cheng, Sheau-Mei J Virol Methods Article A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) method was developed for simultaneous detection and typing/subtyping of influenza viruses A/H1, A/H3 or B, and respiratory syncytial viruses A or B, followed by DNA semiquantitation using the Agilent 2100 Bioanalyzer. Such method provides a rapid, specific and sensitive diagnostic tool for detection and semiquantification of respiratory illness specimens. Elsevier B.V. 2007-01 2006-10-09 /pmc/articles/PMC7112931/ /pubmed/17030067 http://dx.doi.org/10.1016/j.jviromet.2006.08.015 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Chi, X. Sherry
Li, Fenglan
Tam, John S.
Rappaport, Ruth
Cheng, Sheau-Mei
Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title_full Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title_fullStr Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title_full_unstemmed Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title_short Semiquantitative one-step RT-PCR for simultaneous identification of human influenza and respiratory syncytial viruses
title_sort semiquantitative one-step rt-pcr for simultaneous identification of human influenza and respiratory syncytial viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112931/
https://www.ncbi.nlm.nih.gov/pubmed/17030067
http://dx.doi.org/10.1016/j.jviromet.2006.08.015
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