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Monitoring of HIV-1 envelope-mediated membrane fusion using modified split green fluorescent proteins
A simple, cell-based, membrane fusion assay system that uses split green fluorescent proteins (spGFPs) as an indicator was developed. The attachment of the pleckstrin homology (PH) domain to the N-termini of each spGFP not only localized the reporter signal to the plasma membrane but also helped the...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112932/ https://www.ncbi.nlm.nih.gov/pubmed/19559731 http://dx.doi.org/10.1016/j.jviromet.2009.06.017 |