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Electronic Cigarette Aerosol Modulates the Oral Microbiome and Increases Risk of Infection

The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human...

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Detalles Bibliográficos
Autores principales: Pushalkar, Smruti, Paul, Bidisha, Li, Qianhao, Yang, Jian, Vasconcelos, Rebeca, Makwana, Shreya, González, Juan Muñoz, Shah, Shivm, Xie, Chengzhi, Janal, Malvin N., Queiroz, Erica, Bederoff, Maria, Leinwand, Joshua, Solarewicz, Julia, Xu, Fangxi, Aboseria, Eman, Guo, Yuqi, Aguallo, Deanna, Gomez, Claudia, Kamer, Angela, Shelley, Donna, Aphinyanaphongs, Yindalon, Barber, Cheryl, Gordon, Terry, Corby, Patricia, Li, Xin, Saxena, Deepak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113564/
https://www.ncbi.nlm.nih.gov/pubmed/32105635
http://dx.doi.org/10.1016/j.isci.2020.100884
Descripción
Sumario:The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in e-cigarette users influencing the local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, we evaluated 119 human participants, 40 in each of the three cohorts, and found significantly altered beta-diversity in e-cigarette users (p = 0.006) when compared with never smokers or tobacco cigarette smokers. The abundance of Porphyromonas and Veillonella (p = 0.008) was higher among vapers. Interleukin (IL)-6 and IL-1β were highly elevated in e-cigarette users when compared with non-users. Epithelial cell-exposed e-cigarette aerosols were more susceptible for infection. In vitro infection model of premalignant Leuk-1 and malignant cell lines exposed to e-cigarette aerosol and challenged by Porphyromonas gingivalis and Fusobacterium nucleatum resulted in elevated inflammatory response. Our findings for the first time demonstrate that e-cigarette users are more prone to infection.