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Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array

Sendai virus (SeV) is strictly monitored in laboratory rodents. Currently, complete virions have been used as antigens in SeV serological tests. However, the complexity of SeV virion antigen limits the accuracy of the diagnostic method. In the current study, complete SeV virion antigen was separated...

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Autores principales: Xiang, Zhiguang, Tong, Wei, Li, Yuhan, Qin, Chuan, Wei, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113644/
https://www.ncbi.nlm.nih.gov/pubmed/23811230
http://dx.doi.org/10.1016/j.jviromet.2013.06.011
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author Xiang, Zhiguang
Tong, Wei
Li, Yuhan
Qin, Chuan
Wei, Qiang
author_facet Xiang, Zhiguang
Tong, Wei
Li, Yuhan
Qin, Chuan
Wei, Qiang
author_sort Xiang, Zhiguang
collection PubMed
description Sendai virus (SeV) is strictly monitored in laboratory rodents. Currently, complete virions have been used as antigens in SeV serological tests. However, the complexity of SeV virion antigen limits the accuracy of the diagnostic method. In the current study, complete SeV virion antigen was separated on SDS-PAGE and analyzed, with nucleocapsid protein (NP) showing predominant antigenicity. A peptide array containing overlapping 14-mer peptides covering the entire NP was developed. The array used SeV positive serum and resulted in four antigenic linear peptides being identified, which were located in the carboxyl-terminus of NP. The four peptides were coated on ELISA plates and tested with SeV positive and SeV negative sera, and the antigenicity of three peptides, NP413–428, NP473–490 and NP507–524, was confirmed. Mixture of the three peptides showed comparable sensitivity and better specificity in clinical rat sera ELISA tests compared with complete SeV virion antigen. In conclusion, the three peptides, NP413–428, NP473–490 and NP507–524, would be good candidates as linear antigens for SeV detection.
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spelling pubmed-71136442020-04-02 Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array Xiang, Zhiguang Tong, Wei Li, Yuhan Qin, Chuan Wei, Qiang J Virol Methods Article Sendai virus (SeV) is strictly monitored in laboratory rodents. Currently, complete virions have been used as antigens in SeV serological tests. However, the complexity of SeV virion antigen limits the accuracy of the diagnostic method. In the current study, complete SeV virion antigen was separated on SDS-PAGE and analyzed, with nucleocapsid protein (NP) showing predominant antigenicity. A peptide array containing overlapping 14-mer peptides covering the entire NP was developed. The array used SeV positive serum and resulted in four antigenic linear peptides being identified, which were located in the carboxyl-terminus of NP. The four peptides were coated on ELISA plates and tested with SeV positive and SeV negative sera, and the antigenicity of three peptides, NP413–428, NP473–490 and NP507–524, was confirmed. Mixture of the three peptides showed comparable sensitivity and better specificity in clinical rat sera ELISA tests compared with complete SeV virion antigen. In conclusion, the three peptides, NP413–428, NP473–490 and NP507–524, would be good candidates as linear antigens for SeV detection. Elsevier B.V. 2013-11 2013-06-27 /pmc/articles/PMC7113644/ /pubmed/23811230 http://dx.doi.org/10.1016/j.jviromet.2013.06.011 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Xiang, Zhiguang
Tong, Wei
Li, Yuhan
Qin, Chuan
Wei, Qiang
Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title_full Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title_fullStr Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title_full_unstemmed Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title_short Three unique Sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
title_sort three unique sendai virus antigenic peptides screened from nucleocapsid protein by overlapping peptide array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113644/
https://www.ncbi.nlm.nih.gov/pubmed/23811230
http://dx.doi.org/10.1016/j.jviromet.2013.06.011
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