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Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains
A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as...
Autores principales: | Losurdo, Michele, Mari, Viviana, Lucente, Maria Stella, Colaianni, Maria Loredana, Padalino, Iolanda, Cavaliere, Nicola, Buonavoglia, Canio, Decaro, Nicola |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113749/ https://www.ncbi.nlm.nih.gov/pubmed/26300370 http://dx.doi.org/10.1016/j.jviromet.2015.08.013 |
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