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Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus
Feline panleukopenia caused by feline parvovirus (FPV), a single-stranded DNA virus, is typically highly contagious and often presents with lethal syndrome. The broad spectrum of possible hosts suggests its potential for transmission from animal to person through close contact with pets. FPV thus se...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113848/ https://www.ncbi.nlm.nih.gov/pubmed/31216435 http://dx.doi.org/10.1016/j.jviromet.2019.113679 |
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author | Wang, Zhao-Hua Wang, Xiao-Jia Hou, Shao-Hua |
author_facet | Wang, Zhao-Hua Wang, Xiao-Jia Hou, Shao-Hua |
author_sort | Wang, Zhao-Hua |
collection | PubMed |
description | Feline panleukopenia caused by feline parvovirus (FPV), a single-stranded DNA virus, is typically highly contagious and often presents with lethal syndrome. The broad spectrum of possible hosts suggests its potential for transmission from animal to person through close contact with pets. FPV thus serves as an example of the importance of new rapid point-of-care field diagnostic tools for the control and prevention of transmission, especially among rare wild animals and pet cats. Recombinase polymerase amplification (RPA), as a real-time and isothermal method, could be a more affordable alternative to PCR when combined with a lateral flow dipstick (LFD) indicator. In this study, we report a novel FPV lateral flow dipstick RPA (LFD-RPA) instant detection method capable of detecting a range of different FPV strains. The LFD-RPA assay consists of specific primers, probe, and nucleic acid strip. It is capable of detecting 10(2) copies of target nucleic acid per reaction, which is one order of magnitude higher than the sensitivity of traditional PCR. The most suitable reaction conditions for this assay are at 38 ℃ for 15 min. This paper develops an efficient visual detection system that can eliminate the need for professional staff and expensive and sophisticated equipment for field detection. |
format | Online Article Text |
id | pubmed-7113848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71138482020-04-02 Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus Wang, Zhao-Hua Wang, Xiao-Jia Hou, Shao-Hua J Virol Methods Article Feline panleukopenia caused by feline parvovirus (FPV), a single-stranded DNA virus, is typically highly contagious and often presents with lethal syndrome. The broad spectrum of possible hosts suggests its potential for transmission from animal to person through close contact with pets. FPV thus serves as an example of the importance of new rapid point-of-care field diagnostic tools for the control and prevention of transmission, especially among rare wild animals and pet cats. Recombinase polymerase amplification (RPA), as a real-time and isothermal method, could be a more affordable alternative to PCR when combined with a lateral flow dipstick (LFD) indicator. In this study, we report a novel FPV lateral flow dipstick RPA (LFD-RPA) instant detection method capable of detecting a range of different FPV strains. The LFD-RPA assay consists of specific primers, probe, and nucleic acid strip. It is capable of detecting 10(2) copies of target nucleic acid per reaction, which is one order of magnitude higher than the sensitivity of traditional PCR. The most suitable reaction conditions for this assay are at 38 ℃ for 15 min. This paper develops an efficient visual detection system that can eliminate the need for professional staff and expensive and sophisticated equipment for field detection. Elsevier B.V. 2019-09 2019-06-16 /pmc/articles/PMC7113848/ /pubmed/31216435 http://dx.doi.org/10.1016/j.jviromet.2019.113679 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Wang, Zhao-Hua Wang, Xiao-Jia Hou, Shao-Hua Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title | Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title_full | Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title_fullStr | Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title_full_unstemmed | Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title_short | Development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
title_sort | development of a recombinase polymerase amplification assay with lateral flow dipstick for rapid detection of feline parvovirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113848/ https://www.ncbi.nlm.nih.gov/pubmed/31216435 http://dx.doi.org/10.1016/j.jviromet.2019.113679 |
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