New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence o...

Descripción completa

Detalles Bibliográficos
Autores principales: Naguib, Mahmoud M., El-Kady, Magdy F., Lüschow, Dörte, Hassan, Kareem E., Arafa, Abdel-Satar, El-Zanaty, Ali, Hassan, Mohamed K., Hafez, Hafez M., Grund, Christian, Harder, Timm C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113857/
https://www.ncbi.nlm.nih.gov/pubmed/28336367
http://dx.doi.org/10.1016/j.jviromet.2017.02.018
_version_ 1783513759955812352
author Naguib, Mahmoud M.
El-Kady, Magdy F.
Lüschow, Dörte
Hassan, Kareem E.
Arafa, Abdel-Satar
El-Zanaty, Ali
Hassan, Mohamed K.
Hafez, Hafez M.
Grund, Christian
Harder, Timm C.
author_facet Naguib, Mahmoud M.
El-Kady, Magdy F.
Lüschow, Dörte
Hassan, Kareem E.
Arafa, Abdel-Satar
El-Zanaty, Ali
Hassan, Mohamed K.
Hafez, Hafez M.
Grund, Christian
Harder, Timm C.
author_sort Naguib, Mahmoud M.
collection PubMed
description In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1–2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
format Online
Article
Text
id pubmed-7113857
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-71138572020-04-02 New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses Naguib, Mahmoud M. El-Kady, Magdy F. Lüschow, Dörte Hassan, Kareem E. Arafa, Abdel-Satar El-Zanaty, Ali Hassan, Mohamed K. Hafez, Hafez M. Grund, Christian Harder, Timm C. J Virol Methods Article In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1–2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples. Elsevier B.V. 2017-07 2017-03-21 /pmc/articles/PMC7113857/ /pubmed/28336367 http://dx.doi.org/10.1016/j.jviromet.2017.02.018 Text en © 2017 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Naguib, Mahmoud M.
El-Kady, Magdy F.
Lüschow, Dörte
Hassan, Kareem E.
Arafa, Abdel-Satar
El-Zanaty, Ali
Hassan, Mohamed K.
Hafez, Hafez M.
Grund, Christian
Harder, Timm C.
New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title_full New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title_fullStr New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title_full_unstemmed New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title_short New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses
title_sort new real time and conventional rt-pcrs for updated molecular diagnosis of infectious bronchitis virus infection (ibv) in chickens in egypt associated with frequent co-infections with avian influenza and newcastle disease viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113857/
https://www.ncbi.nlm.nih.gov/pubmed/28336367
http://dx.doi.org/10.1016/j.jviromet.2017.02.018
work_keys_str_mv AT naguibmahmoudm newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT elkadymagdyf newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT luschowdorte newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT hassankareeme newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT arafaabdelsatar newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT elzanatyali newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT hassanmohamedk newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT hafezhafezm newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT grundchristian newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses
AT hardertimmc newrealtimeandconventionalrtpcrsforupdatedmoleculardiagnosisofinfectiousbronchitisvirusinfectionibvinchickensinegyptassociatedwithfrequentcoinfectionswithavianinfluenzaandnewcastlediseaseviruses

Ejemplares similares