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Rapid manipulation of the porcine epidemic diarrhea virus genome by CRISPR/Cas9 technology
Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic enteric coronavirus causing lethal watery diarrhea in suckling piglets. Reverse genetics is a valuable tool to study the functions of viral genes and to generate vaccine candidates. In this study, a full-length infectious cDNA clone of th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113866/ https://www.ncbi.nlm.nih.gov/pubmed/31712093 http://dx.doi.org/10.1016/j.jviromet.2019.113772 |