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Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus
Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was develop...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113876/ https://www.ncbi.nlm.nih.gov/pubmed/25887451 http://dx.doi.org/10.1016/j.jviromet.2015.04.008 |
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author | Yuan, Wanzhe Li, Yanan Li, Peng Song, Qinye Li, Limin Sun, Jiguo |
author_facet | Yuan, Wanzhe Li, Yanan Li, Peng Song, Qinye Li, Limin Sun, Jiguo |
author_sort | Yuan, Wanzhe |
collection | PubMed |
description | Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10(−6) ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection. |
format | Online Article Text |
id | pubmed-7113876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71138762020-04-02 Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus Yuan, Wanzhe Li, Yanan Li, Peng Song, Qinye Li, Limin Sun, Jiguo J Virol Methods Short Communication Porcine epidemic diarrhea virus (PEDV) is an important pig pathogen that can cause vomiting, diarrhea, and dehydration, leading to serious damage to the swine industry worldwide. In this study, a nanoparticle-assisted polymerase chain reaction (nanoPCR) assay targeting the N gene of PEDV was developed and the sensitivity and specificity were investigated. Under the optimized conditions for detection of PEDV RNA, the nanoPCR assay was 100-fold more sensitive than a conventional RT-PCR assay. The lower detection limit of the nanoPCR assay was 2.7 × 10(−6) ng/μL of PEDV RNA and no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of PEDV. The sensitive and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of PEDV-infection. Elsevier B.V. 2015-08 2015-04-14 /pmc/articles/PMC7113876/ /pubmed/25887451 http://dx.doi.org/10.1016/j.jviromet.2015.04.008 Text en Copyright © 2015 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Yuan, Wanzhe Li, Yanan Li, Peng Song, Qinye Li, Limin Sun, Jiguo Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title | Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title_full | Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title_fullStr | Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title_full_unstemmed | Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title_short | Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus |
title_sort | development of a nanoparticle-assisted pcr assay for detection of porcine epidemic diarrhea virus |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113876/ https://www.ncbi.nlm.nih.gov/pubmed/25887451 http://dx.doi.org/10.1016/j.jviromet.2015.04.008 |
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