Cargando…

Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy

BACKGROUND AND AIMS: Recurrent Pregnancy loss (RPL) is a heterogeneous disease. The role of maternal factor is clear but the relationship between the paternal factors remains uncertain. It has been shown that increase the level of Reactive Oxygen Species (ROS) and decrease the antioxidant levels in...

Descripción completa

Detalles Bibliográficos
Autores principales: Nazari, Alireza, Sabeti, Parvin, Pourmasumi, Soheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113919/
https://www.ncbi.nlm.nih.gov/pubmed/32318388
http://dx.doi.org/10.4103/jfmpc.jfmpc_1105_19
_version_ 1783513771057086464
author Nazari, Alireza
Sabeti, Parvin
Pourmasumi, Soheila
author_facet Nazari, Alireza
Sabeti, Parvin
Pourmasumi, Soheila
author_sort Nazari, Alireza
collection PubMed
description BACKGROUND AND AIMS: Recurrent Pregnancy loss (RPL) is a heterogeneous disease. The role of maternal factor is clear but the relationship between the paternal factors remains uncertain. It has been shown that increase the level of Reactive Oxygen Species (ROS) and decrease the antioxidant levels in men can lead to RPL. New researches show treatment with antioxidant can improved sperm parameter. The aim of the present study was to assess the effect of vitamin E plus Zinc therapy on sperm parameters and chromatin quality in couples with RPL. METHODS: In this clinical trial study, 60 RPL patients were selected from RPL clinic. Standard sperm parameters were analyzed and then male partners were intake vitamin E and Zinc in antioxidant therapy plan for 90 days. After that, sperm parameters were analyzed again. Sperm chromatin assay was reviewed before and after vitamin E and Zinc therapy by cytochemical assay including aniline blue (AB), chromomycin A3 (CMA3), toluidine blue (TB). To assess DNA fragmentation index, TUNEL test was used. Data were analyzed and compared before and after treatment. RESULTS: data analysis showed all sperm parameters significantly improved after treatment (P < 0.001). The number of AB(+) and TB(+) sperms were decreased significantly after vitamin E and Zinc therapy (P = 0.0001). Decrease in DNA fragmentation in post treatment group in comparison to pre-treatment was statistically significant. CONCLUSION: Supplemental of vitamin E plus Zinc may improve sperm parameters chromatin quality and decrease sperm DNA fragmentation in RPL patients.
format Online
Article
Text
id pubmed-7113919
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-71139192020-04-21 Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy Nazari, Alireza Sabeti, Parvin Pourmasumi, Soheila J Family Med Prim Care Original Article BACKGROUND AND AIMS: Recurrent Pregnancy loss (RPL) is a heterogeneous disease. The role of maternal factor is clear but the relationship between the paternal factors remains uncertain. It has been shown that increase the level of Reactive Oxygen Species (ROS) and decrease the antioxidant levels in men can lead to RPL. New researches show treatment with antioxidant can improved sperm parameter. The aim of the present study was to assess the effect of vitamin E plus Zinc therapy on sperm parameters and chromatin quality in couples with RPL. METHODS: In this clinical trial study, 60 RPL patients were selected from RPL clinic. Standard sperm parameters were analyzed and then male partners were intake vitamin E and Zinc in antioxidant therapy plan for 90 days. After that, sperm parameters were analyzed again. Sperm chromatin assay was reviewed before and after vitamin E and Zinc therapy by cytochemical assay including aniline blue (AB), chromomycin A3 (CMA3), toluidine blue (TB). To assess DNA fragmentation index, TUNEL test was used. Data were analyzed and compared before and after treatment. RESULTS: data analysis showed all sperm parameters significantly improved after treatment (P < 0.001). The number of AB(+) and TB(+) sperms were decreased significantly after vitamin E and Zinc therapy (P = 0.0001). Decrease in DNA fragmentation in post treatment group in comparison to pre-treatment was statistically significant. CONCLUSION: Supplemental of vitamin E plus Zinc may improve sperm parameters chromatin quality and decrease sperm DNA fragmentation in RPL patients. Wolters Kluwer - Medknow 2020-02-28 /pmc/articles/PMC7113919/ /pubmed/32318388 http://dx.doi.org/10.4103/jfmpc.jfmpc_1105_19 Text en Copyright: © Journal of Family Medicine and Primary Care http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Nazari, Alireza
Sabeti, Parvin
Pourmasumi, Soheila
Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title_full Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title_fullStr Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title_full_unstemmed Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title_short Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
title_sort comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113919/
https://www.ncbi.nlm.nih.gov/pubmed/32318388
http://dx.doi.org/10.4103/jfmpc.jfmpc_1105_19
work_keys_str_mv AT nazarialireza comparisonbetweenspermparametersandchromatininrecurrentpregnancylosscouplesafterantioxidanttherapy
AT sabetiparvin comparisonbetweenspermparametersandchromatininrecurrentpregnancylosscouplesafterantioxidanttherapy
AT pourmasumisoheila comparisonbetweenspermparametersandchromatininrecurrentpregnancylosscouplesafterantioxidanttherapy